2024-03-29T13:12:04Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/710592022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141Availability of Nanopore sequences in the genome taxonomy for Vibrionaceae systematics: Rumoiensis Glade species as a test caseTanaka, MamiMino, SayakaOgura, YoshitoshiHayashi, TetsuyaSawabe, TomooVibrionaceaeGenome taxonomyNanoporeWhole genome sequence comparisons have become essential for establishing a robust scheme in bacterial taxonomy. To generalize this genome-based taxonomy, fast, reliable, and cost-effective gnome sequencing methodologies are required. MinION, the palm -sized sequencer from Oxford Nanopore Technologies, enables rapid sequencing of bacterial genomes using minimal laboratory resources. Here we tested the ability of Nanopore sequences for the genome-based taxonomy of Vibrionaceae and compared Nanopore- only assemblies to complete gnomes of fiw Rumoiensis clade species: Vibrio aphrogenes, V. algivorus, V. casei, V. litoralis, and V. rumoiensis. Comparison of overall gnome relatedness indices (OGRI) and multilocus sequence analysis (MLSA) based on Nanopore-only assembly and Illumina or hybrid assemblies revealed that errors in Nanopore-only assembly do not Influence average nucleotide identity (ANI), in silico DNA -DNA hybridization (DDH), G+C content, or MLSA tree topology in Vibrionaceae. Our results show that the genome sequences frorn Nanopore-based approach can be used for rapid species identification based on the OGRI and MLSA.PeerJJournal Articleapplication/pdfhttp://hdl.handle.net/2115/71059https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71059/1/peerj-5018.pdf2167-8359Peerj6e50182018-06-18enginfo:doi/10.7717/peerj.5018https://creativecommons.org/licenses/by/4.0/publisher