2024-03-29T07:12:46Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/711422023-10-05T04:30:10Zhdl_2115_20056hdl_2115_147Desulfocucumis palustris gen. nov., sp nov., a mesophilic sulfate reducer belonging to Desulfotomaculum subcluster IgWatanabe, MihoKojima, HisayaFukui, ManabufirmicutesDesulfotomaculumsulfate-reducing bacterium470A mesophilic, endospore-forming, sulfate-reducing bacterium, designated strain NAW-5(T), was isolated from marsh soil. Cells of strain NAW-5(T) were Gram-stain-negative, curved rods that were motile. Strain NAW-5(T) grew at 18-48 degrees C (optimum 3237 degrees C) and pH 5.8-8.4 (optimum pH 6.2-7.3). Electron donors utilized were various organic acids and H-2 which support autotrophic growth. Fermentative growth occurred on carboxylic acids, but not on sugar. Sulfate, thiosulfate and elemental sulfur were used as electron acceptors. The respiratory isoprenoid quinone was MK-7. The genomic DNA G+C content of this strain was 46.6 mol%. Sequence analysis of the 16S rRNA gene showed that strain NAW-5(T) was affiliated to the family 'Desulfotomacul aceae' but the strain shared very low sequence similarity with any representatives of this family (>= 89 %). Strain NAW-5(T) belongs to Desulfotomaculum subcluster Ig which does not include any species with validly published names. On the basis of significant differences in the phylogenetic and phenotypic properties between strain NAW-5(T) and related species, strain NAW-5(T) represents a novel species of a new genus for which the name Desulfocucumis palustris gen. nov., sp. nov. is proposed. The type strain of the type species is NAW-5(T) (= DSM 102911(T) = NBRC 112242(T)).The Microbiology SocietyJournal Articleapplication/pdfapplication/pdfhttp://hdl.handle.net/2115/71142https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71142/1/NAW-5_manuscript_170403.pdfhttps://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71142/2/supplementaly%20Fig.%20S1.pdf1466-5026International journal of systematic and evolutionary microbiology678267926822017-08-01enginfo:pmid/28786781info:doi/10.1099/ijsem.0.002005author