2024-03-28T13:33:23Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/715632022-11-17T02:08:08Zhdl_2115_35410hdl_2115_35409Profiling of cardiolipins and their hydroperoxides in HepG2 cells by LC/MSChen, ZhenWu, YueMa, Yi-ShingKobayashi, YuuZhao, Yao-YaoMiura, YusukeChiba, HitoshiHui, Shu-PingCardiolipinLipid hydroperoxidesCL-OOHMolecular speciesMitochondriaLC-HR-MS/MS490Cardiolipin (CL) exists as crucial functional phospholipid in mitochondria. The oxidation of CL is concerned with mitochondrial dysfunction and various diseases. As main oxidation products, CL hydroperoxide (CL-OOH) plays a key role in intermediating oxidative reaction. Thus, direct analysis of CL-OOH is of great interest. In the present study, CL and CL-OOH profiles were analyzed in oxidized HepG2 cell lipid via HPLC-Orbitrap MS/MS. Furthermore, the contents of individual molecular species were compared between intact and AAPH-oxidized HepG2 cells. In total, 46 CL and 18 CL-OOH were identified from oxidized cell lipids, while 21 CL and 9 CL-OOH were detected in AAPH-treated cells. Most CL depleted significantly after AAPH inducement, with percentages varying from 8.3% (CL70:7) to 73.7% (CL72:4), depending on fatty acyl composition. While almost all the CL-OOH remarkably increased, among them 68:6-, 72:6-, and 72:7-OOHs were only detected in AAPH-treated cells. CL68:5- and CL68:4-OOH were the most abundant species, while CL70:5-OOH among all the species expressed the highest oxidation percentage of the corresponding CL. Our results showed practical separation, identification, and semi-quantitation of CL-OOH species, which could contribute to approaches to lipidomic analysis of CL and CL-OOH, as well as tracing biomarkers in mitochondrial oxidative stress diagnosis.SpringerJournal Articleapplication/pdfhttp://hdl.handle.net/2115/71563https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71563/1/Anal.%20Bioanal.%20Chem_409%2824%29_5735-5745.pdf1618-26421618-2650Analytical and bioanalytical chemistry40924573557452017-09enginfo:pmid/28762068info:doi/10.1007/s00216-017-0515-3The final publication is available at link.springer.com via http://dx.doi.org/10.1007/s00216-017-0515-3.author