2024-03-28T19:48:39Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/717012022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid .GAMMA.-Lactone ChemiluminescenceKAMIDATE, TamioKIKUCHI, NobuhitoISHIDA, AkihikoTANI, HirofumiHomogentisic acid γ-lactone (HAL)chemiluminescence (CL)determination of horseradish peroxidase (HRP)Homogentisic acid γ-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP was detected after the lysis of HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range of calibration curve for HRP in HAL CL were compared with those in p-iodophenol (p-IP)-enhanced luminol CL. Maximal light emission in HAL CL appeared more rapidly compared to that in p-IP enhanced luminol CL, thus resulting in remarkable reduction of CL measurement time. The detection limit for HRP in HAL CL was the same as that in p-IP-enhanced luminol CL. The linear range of calibration curve for HRP in HAL CL was improved by a factor of 50 compared with that in p-IP-enhanced luminol CL. From these results, it was found that HAL CL were superior to p-IP-enhanced luminol CL for the determination of HRP encapsulated in liposomes.日本分析化学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/71701https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71701/1/Anal.sci.21-701.pdf0910-63401348-2246AA10500785Analytical Sciences2167017042005-06enginfo:doi/10.2116/analsci.21.701publisher