2024-03-28T21:01:05Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/717122022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139Uptake of Transition Metal Ions Using Liposomes Containing Dicetylphosphate as a LigandKAMIDATE, TamioHASHIMOTO, YasunoriTANI, HirofumiISHIDA, Akihikophosphatidylcholine (PC)The uptake of Cu2+ was investigated using various types of liposomes composed of phosphatidylcholine (PC), cholesterol (Chol) and dicethylphosphate (DCP). DCP played a role as a ligand for Cu2+. Multilamellar vesicles (MLVs) were more effective for the uptake of Cu2+ compared to unilamellar vesicles prepared by the extrusion technique. The uptake efficiency of MLVs for Cu2+ was dependent on the molar ratio of DCP in MLVs. The uptake percent of Cu2+ was 92% using MLVs having a PC:DCP:Chol molar ratio of 4:3:3; 95% of the total vesicle Cu2+ was bound to DCP of the outer membrane surface of the MLVs, and the remaining 5% of the total Cu2+ was distributed into the interior side of the MLVs. MLVs having a PC:DCP:Chol molar ratio of 4:3:3 were also effective as separation media for Mn2+, Co2+, Ni2+ and Zn2+. The uptake efficiency of the MLVs for the transition-metal ions increased in the order Co2+< Zn2+ < Ni2+ < Mn2+ < Cu2+.日本分析化学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/71712https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71712/1/Anal.sci.18-273.pdf0910-63401348-2246AA10500785Analytical Sciences1832732762002-03enginfo:doi/10.2116/analsci.18.273publisher