2024-03-29T09:13:50Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/717382022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139A Portable Liquid Chromatograph with a Battery-operated Compact Electroosmotic Pump and a Microfluidic Chip Device with a Reversed Phase Packed ColumnISHIDA, AkihikoFUJII, MitsutakaFUJIMOTO, TakehiroSASAKI, ShunsukeYANAGISAWA, IchiroTANI, HirofumiTOKESHI, Manabuelectroosmotic pumpliquid chromatographymicrofluidic chippacked column433A compact and lightweight liquid chromatography system is presented with overall dimensions of 26 cm width × 18 cm length × 21 cm height and weight of 2 kg. This system comprises a battery-operated compact electroosmotic pump, a manual injector, a microfluidic chip device containing a packed column and an electrochemical detector, and a USB bus-powered potentiostat. The pumping system was designed for microfluidic-based reversed-phase liquid chromatography in which an electroosmotically generated water stream pushes the mobile phase via a diaphragm for the output. The flow rate ranged from 0 to 10 μL/min and had a high degree of precision. The pumping system operated continuously for over 24 h with dry batteries. The column formed in the microfluidic device was packed with 3-μm ODS particles with a length of 30 mm and a diameter of 0.8 mm. The results presented herein demonstrate the performance of the pumping system and the column using alkylphenols, catecholamine, catechin, and amino acids.日本分析化学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/71738https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71738/1/Anal.sci.31-1163.pdf0910-63401348-2246AA10500785Analytical Sciences3111116311692015-11-10enginfo:pmid/26561261info:doi/10.2116/analsci.31.1163publisher