2024-03-28T23:57:23Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/717442022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139Microfluidic Approaches for Protein Crystal Structure AnalysisMAEKI, MasatoshiYAMAGUCHI, HiroshiTOKESHI, ManabuMIYAZAKI, Masayamicrofluidic deviceprotein crystallizationprotein crystallographyin-situ X-ray analysis433This review summarizes two microfluidic-based protein crystallization methods, protein crystallization behavior in the microfluidic devices, and their applications for X-ray crystal structure analysis. Microfluidic devices provide many advantages for protein crystallography; they require small sample volumes, provide high-throughput screening, and allow control of the protein crystallization. A droplet-based protein crystallization method is a useful technique for high-throughput screening and the formation of a single crystal without any complicated device fabrication process. Well-based microfluidic platforms also enable effective protein crystallization. This review also summarizes the protein crystal growth behavior in microfluidic devices as, is known from viewpoints of theoretical and experimental approaches. Finally, we introduce applications of microfluidic devices for on-chip crystal structure analysis.日本分析化学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/71744https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/71744/1/Anal.sci.32-3.pdf0910-63401348-2246AA10500785Analytical Sciences321392016-01-10enginfo:pmid/26753699info:doi/10.2116/analsci.32.3publisher