2024-03-29T01:25:14Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/723352022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139Roles of Microbial Activity and Anthraquinone-2,7-disulfonate as a Model of Humic Substances in Leaching of Iron from Hematite into SeawaterAneksampant, ApichayaTanaka, AtsushiTu, XuefeiIwai, HisanoriYamamoto, MitsuoNakashima, KazunoriFukushima, MasamiAnthraquinone-2,7-disulfonatebarren groundExiguobacterium oxidotolerans sp.hematiteiron elutioniron reductionseawater519Fertilization with a mixture of steelmaking slag and compost can affect the supply of dissolved iron used to restore seaweed beds, however, the mechanisms of iron elution from the fertilizer are not well understood. In the present study, the microorganism was isolated from Fe-fertilizer incubated in coastal seawater for 6 months, and was identified as Exiguobacterium oxidotolerans by 16S rDNA sequencing. The iron elutability of the bacteria was proved based on the increasing of dissolved iron by incubation with Fe2O3 (hematite) under a seawater-like condition. The value of ORP was changed by inoculated of the bacteria from ca. 0 to ca. -400 mV, which is anticipated concerning to reduction of Fe. The concentration of eluted iron was largely depended on those of organic acids produced by bacteria. From the results, it was proved that E. oxidotolerans is capable of Fe reductive eluting of iron from Fe2O3 into seawater. Anthraquinone-2,7-disulfonate (AQDS), which can play as an electron acceptor/donor between microbe and insoluble Fe2O3 particles, enhanced the effect of iron bio-leaching.日本分析化学会Journal Articleapplication/pdfhttp://hdl.handle.net/2115/72335https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/72335/3/34_18P147.pdf0910-6340Analytical sciences3411130313082018-11enginfo:pmid/30078814info:doi/10.2116/analsci.18P147publisher