2024-03-29T15:42:48Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/727202022-11-17T02:08:08Zhdl_2115_20045hdl_2115_139A compact fluorescence polarization analyzer with high-transmittance liquid crystal layerWakao, OsamuSatou, KenNakamura, AyanoSumiyoshi, KenShirokawa, MasanoriMizokuchi, ChikaakiShiota, KunihiroMaeki, MasatoshiIshida, AkihikoTani, HirofumiShigemura, KojiHibara, AkihideTokeshi, ManabuFluorescence polarization (FP) offers easy operation and rapid processing, making it implementable in molecular interaction analysis. Previously we have developed a unique FP measurement system using a liquid crystal (LC) layer and an image sensor. The system is based on a principle of synchronized detection between the switching rate of the LC layer and the sampling rate of the CCD. The FP system realized simultaneous multiple sample detection; however, the measurement precision was lower than that of the conventional FP apparatus. The main drawbacks were low light transmittance of the LC layer and insufficient synchronization between the LC layer and CCD. In this paper, we developed a new FP analyzer based on LC-CCD synchronization detection. By using a newly designed LC with high transmittance and improving synchronization, the performance of the system has been dramatically improved. Additionally, we reduced the cost by using an inexpensive CCD and an LED as the excitation source. Simultaneous FP immunoassay of multiple samples of prostaglandin E2 was performed. The error rate of the FP system is reduced from 16.9% to 3.9%, as comparable to the commercial conventional FP system. Published by AIP Publishing.American Institute of Physics (AIP)Journal Articleapplication/pdfhttp://hdl.handle.net/2115/72720https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/72720/1/1.5017081.pdf0034-6748AA00817730Review of Scientific Instruments8920241032018-02-27enginfo:pmid/29495833info:doi/10.1063/1.5017081This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Review of Scientific Instruments 89, 024103 (2018) and may be found at https://doi.org/10.1063/1.5017081.publisher