2024-03-29T14:08:08Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/753512022-11-17T02:08:08Zhdl_2115_20044hdl_2115_124MicroRNA-101-5p Suppresses the Expression of the Ras-Related Protein RAP1AShibayama, YoshihikoKubo, YoshitadaNakagawa, TsutomuIseki, Kenfocal adhesion kinase (FAK)proliferationRacRAS family proteinsalirasib460MicroRNAs (miRs) are small, non-coding RNAs that negatively regulate gene expression. The stem loop sequence miR-101-1 generates mature miR-101-5p and miR-101-3p. The function and target mRNA of miR-101-5p have not yet been elucidated in detail. Here, we demonstrate that miR-101-5p inhibits the expression of RAP1A, a member of the RAS gene family. Transfection of a miR-101-5p mimic significantly inhibited the expression of RAP1A mRNA in HeLa, HEK293, A549, and COLO201 cells. The same treatment significantly inhibited cell proliferation. The cytostatic effect with transfection of miR-101-5p was antagonized by treatment with the RAP inhibitor salirasib. These results suggested that miR-101-5p inhibits RAP1A, and thus, the expression levels of miR-101-5p regulate cell proliferation.The Pharmaceutical Society of JapanJournal Articlehttp://hdl.handle.net/2115/753510918-6158Biological & pharmaceutical bulletin428133213362019-08enginfo:doi/10.1248/bpb.b19-00068none