2024-03-29T14:41:07Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/754822022-11-17T02:08:08Zhdl_2115_20049hdl_2115_141Factors influencing the induction of adventitious bud and callus in the brown alga Sargassum horneri (Turner) C. AgardhUji, ToshikiNanaumi, DaisukeKawagoe, ChikaraSaga, NaotsuneMiyashita, KazuoBrown algaClonal propagationExplant cultureSeed materialPlant growth regulatorsUniconazole474A high frequency of callus induction and propagation from leaf explants of the brown alga Sargassum horneri was achieved within 2 months of culture when grown in medium supplemented with 5 μM uniconazole, which is a triazole-type inhibitor of cytochrome P450 enzymes. Adventitious buds were efficiently formed from the pigmented callus after transfer to medium without uniconazole, indicating that treatment with uniconazole was more beneficial for regeneration of the alga. Favorable culture conditions for induction of adventitious buds and calli included temperatures of 15 to 25 °C and light levels of 20 to 200 μmol photons m−2 s−1. Blue light promoted the production of adventitious buds and calli. The frequency of formation of adventitious buds and calli in explants from thalli with one leaf was more than 90 %, while it was 10 % only when explants were sourced from thalli with 9 to 11 leaves. These findings will be useful for clonal propagation and storage of seed materials for mariculture of Sargassum species.SpringerJournal Articleapplication/pdfhttp://hdl.handle.net/2115/75482https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/75482/1/JAPH%20revised%202015.10.pdf0921-89711573-5176Journal of Applied Phycology284243524432016-08enginfo:doi/10.1007/s10811-015-0745-8The final publication is available at link.springer.comauthor