2024-03-29T09:12:36Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/760012022-11-17T02:08:08Zhdl_2115_20048hdl_2115_140An experimental study of menopause induced by bilateral ovariectomy and mechanistic effects of mesenchymal stromal cell therapy on the parotid gland of a rat modelEl-naseery, Nesma IbraheimElewa, Yaser Hosny AliIchii, OsamuKon, YasuhiroParotid glandOvariectomizedMesenchymal stromal cellsImmunohistochemistry649The current study was conducted on a menopause rat model induced by ovariectomy to assess the histological and immunohistochemical alterations in the parotid glands and to verify the efficiency of human umbilical cord derived-mesenchymal stromal cell (hUCB-MSCs) in treating this condition. Eighteen adult female rats were equally divided into three groups: sham-operated (SHAM), ovariectomized (OVX) and OVX injected with hUCB-MSCs (OVX + hUCB-MSCs). At 3 months post-ovariectomy, the salivary flow rate and size of the parotid glands were measured. The parotid glands were histologically investigated via H&E stained sections. Furthermore, immunohistochemical analysis for human CD105, human CD34, proliferating cell nuclear antigen (PCNA), single strand DNA (ssDNA), caspase 3, aquaporin (AQP)1, alpha-smooth muscle actin (alpha-SMA) and mouse CD34 were performed. The OVX group showed interstitial hemorrhage, dispersed acini and intracytoplasmic vacuoles in the acinar cells. Furthermore, immunohistochemical staining revealed a significant decrement in the number of ssDNA positive apoptotic cells, but a significant increment of PCNA positive proliferating cells, AQP1 positive blood capillaries, alpha-SMA positive myoepithelial cells and endogenous CD34 positive hematopoietic progenitor cells in the OVX + hUCBMSCs group as compared with the OVX group. These findings suggest a potential regenerative therapy of MSCs to injured parotid gland structures. However, further investigations are required to illustrate the mechanism of hUCB-MSCs mediated parotid gland regeneration. (C) 2018 Elsevier GmbH. All rights reserved.ElsevierJournal Articleapplication/pdfhttp://hdl.handle.net/2115/76001https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/76001/1/AANAT2786R2.pdf0940-9602Annals of anatomy - Anatomischer anzeiger2209202018-11enginfo:doi/10.1016/j.aanat.2018.06.006© 2018. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/author