2024-03-28T17:13:43Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/790222022-11-17T02:08:08Zhdl_2115_20058hdl_2115_149LncRNA-dependent nuclear stress bodies promote intron retention through SR protein phosphorylationNinomiya, KensukeAdachi, ShungoNatsume, TohruIwakiri, JunichiTerai, GoroAsai, KiyoshiHirose, Tetsurointron retentionnoncoding RNAnuclear stress bodiesphosphorylationsplicing factors490A number of long noncoding RNAs (lncRNAs) are induced in response to specific stresses to construct membrane-less nuclear bodies; however, their function remains poorly understood. Here, we report the role of nuclear stress bodies (nSBs) formed on highly repetitive satellite III (HSATIII) lncRNAs derived from primate-specific satellite III repeats upon thermal stress exposure. A transcriptomic analysis revealed that depletion of HSATIII lncRNAs, resulting in elimination of nSBs, promoted splicing of 533 retained introns during thermal stress recovery. A HSATIII-Comprehensive identification of RNA-binding proteins by mass spectrometry (ChIRP-MS) analysis identified multiple splicing factors in nSBs, including serine and arginine-rich pre-mRNA splicing factors (SRSFs), the phosphorylation states of which affect splicing patterns. SRSFs are rapidly de-phosphorylated upon thermal stress exposure. During stress recovery, CDC like kinase 1 (CLK1) was recruited to nSBs and accelerated the re-phosphorylation of SRSF9, thereby promoting target intron retention. Our findings suggest that HSATIII-dependent nSBs serve as a conditional platform for phosphorylation of SRSFs by CLK1 to promote the rapid adaptation of gene expression through intron retention following thermal stress exposure.John Wiley & SonsJournal Articleapplication/pdfhttp://hdl.handle.net/2115/79022https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/79022/1/EMBOJ-2019-102729R1_Merged_PDF.pdf0261-4189EMBO Journal3923e1027292020-02-03enginfo:doi/10.15252/embj.2019102729This is the peer reviewed version of the following article: The EMBO Journal: 39(3): e102729., which has been published in final form at https://doi.org/10.15252/embj.2019102729. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.author