2024-03-29T13:56:54Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/805662022-11-17T02:08:08Zhdl_2115_20076hdl_2115_597TMPRSS11D and TMPRSS13 Activate the SARS-CoV-2 Spike ProteinKishimoto, MaiUemura, KentaroSanaki, TakaoSato, AkihikoHall, William W.Kariwa, HiroakiOrba, YasukoSawa, HirofumiSasaki, Michihitosevere acute respiratory syndrome-like coronavirus-2 (SARS-CoV-2)type II transmembrane serine protease (TTSP)spike protein490Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) utilizes host proteases, including a plasma membrane-associated transmembrane protease, serine 2 (TMPRSS2) to cleave and activate the virus spike protein to facilitate cellular entry. Although TMPRSS2 is a well-characterized type II transmembrane serine protease (TTSP), the role of other TTSPs on the replication of SARS-CoV-2 remains to be elucidated. Here, we have screened 12 TTSPs using human angiotensin-converting enzyme 2-expressing HEK293T (293T-ACE2) cells and Vero E6 cells and demonstrated that exogenous expression of TMPRSS11D and TMPRSS13 enhanced cellular uptake and subsequent replication of SARS-CoV-2. In addition, SARS-CoV-1 and SARS-CoV-2 share the same TTSPs in the viral entry process. Our study demonstrates the impact of host TTSPs on infection of SARS-CoV-2, which may have implications for cell and tissue tropism, for pathogenicity, and potentially for vaccine development.MDPIJournal Articleapplication/pdfhttp://hdl.handle.net/2115/80566https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/80566/1/viruses-13-00384-v2.pdf1999-4915Viruses1333842021-03enginfo:pmid/33671076info:doi/10.3390/v13030384https://creativecommons.org/licenses/by/4.0/publisher