2024-03-29T06:54:49Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/816972022-11-17T02:08:08Zhdl_2115_20058hdl_2115_149Disinfectant potential in inactivation of epidemic keratoconjunctivitis-related adenoviruses by potassium peroxymonosulfateHashizume, MeiAoki, KokiOhno, ShigeakiKitaichi, NobuyoshiYawata, NobuyoGonzalez, GabrielNonaka, HirotakaSato, SeiichiTakaoka, AkinoriHuman adenovirusepidemic keratoconjunctivitisdisinfectantspreventionRUBYSTAVirkon490Purpose: The aim of this study was to test the antiviral effectivity of potassium peroxymonosulfate (RUBYSTA (R), KYORIN) against five epidemic keratoconjunctivitis-related types of Human adenovirus D in vitro. Methods: Five types of Human adenovirus D (8, 37, 53, 54 and 56) were incubated with 1% potassium peroxymonosulfate, 0.1% sodium hypochlorite (NaClO) or alcohol-based disinfectant for 30 s or 1 min. These solutions were subjected to measurements of viral titres by infection assays in A549 cells. At day 6 post-infection, both, supernatants and cells, were collected and the viral genome was assessed by real-time polymerase chain reaction analysis. Results: Treatments with 1% potassium peroxymonosulfate led to significant reduction in all tested Human adenovirus D types comparable to disinfecting effects by 0.1% NaClO. Overall, potassium peroxymonosulfate demonstrated sufficient inactivation of the major epidemic keratoconjunctivitis-causing Human adenovirus D to be considered for disinfection and prevention purposes in ophthalmological clinics and hospitals. Conclusion: This study demonstrated that potassium peroxymonosulfate is a promising disinfectant for the prevention of epidemic keratoconjunctivitis nosocomial infections in ophthalmological clinics.SAGE PublicationsJournal Articleapplication/pdfhttp://hdl.handle.net/2115/81697https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/81697/1/TEXT_MH_EJO_final.pdf1120-6721European journal of ophthalmology3123793842021-03enginfo:doi/10.1177/1120672119891408Mei Hashizume, Koki Aoki, Shigeaki Ohno, Nobuyoshi Kitaichi, Nobuyo Yawata, Gabriel Gonzalez, Hirotaka Nonaka, Seiichi Sato, Akinori Takaoka, Disinfectant potential in inactivation of epidemic keratoconjunctivitis-related adenoviruses by potassium peroxymonosulfate Show less, European Journal of Ophthalmology Vol. 31(2) pp. 379-384. Copyright ©2021 (Copyright Holder). DOI:10.1177/1120672119891408author