2024-03-28T18:17:07Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/819592022-11-17T02:08:08Zhdl_2115_20048hdl_2115_140The enhancement of Th1 immune response by anti-PD-L1 antibody in cattle infected with Mycobacterium avium subsp. paratuberculosisSajiki, YamatoKonnai, SatoruNagata, ReikoKawaji, SatokoNakamura, HayatoFujisawa, SotaroOkagawa, TomohiroMaekawa, NaoyaKato, YukinariSuzuki, YasuhikoMurata, ShiroMori, YasuyukiOhashi, KazuhikocattleimmunotherapyJohne's diseaseprogrammed death-ligand 1Th1 response460Johne's disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is a chronic enteritis of ruminants. Previous studies have shown that programmed death-ligand 1 (PD-L1) is associated with the disease progression, and PD-L1 blockade activates MAP-specific Th1 responses in vitro. Here, we performed anti-PD-L1 antibody administration using 2 MAP-infected cattle at the late subclinical stage of infection. After administration, bacterial shedding was reduced or maintained at a low level. Additionally, MAP-specific Th1 cytokine production was upregulated, and CD69 expression was increased in T cells. Collectively, the treatment has a potential as a novel control method against Johne's disease.公益社団法人 日本獣医学会 (The Japanese Society of Veterinary Science)Journal Articlehttp://hdl.handle.net/2115/819590916-7250Journal of veterinary medical science8321621662021-02enginfo:doi/10.1292/jvms.20-0590none