2024-03-29T02:32:21Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/823852022-11-17T02:08:08Zhdl_2115_20048hdl_2115_140Thymoquinone and Curcumin Defeat Aging-Associated Oxidative Alterations Induced by D-Galactose in Rats' Brain and HeartEl-Far, Ali H.Elewa, Yaser H. A.Abdelfattah, Elsayeda-Zeinab A.Alsenosy, Abdel-Wahab A.Atta, Mustafa S.Abou-Zeid, Khalid M.Al Jaouni, Soad K.Mousa, Shaker A.Noreldin, Ahmed E.thymoquinonecurcuminD-galactoseoxidative stressanti-aging460D-galactose (D-gal) administration causes oxidative disorder and is widely utilized in aging animal models. Therefore, we subcutaneously injected D-gal at 200 mg/kg BW dose to assess the potential preventive effect of thymoquinone (TQ) and curcumin (Cur) against the oxidative alterations induced by D-gal. Other than the control, vehicle, and D-gal groups, the TQ and Cur treated groups were orally supplemented at 20 mg/kg BW of each alone or combined. TQ and Cur effectively suppressed the oxidative alterations induced by D-gal in brain and heart tissues. The TQ and Cur combination significantly decreased the elevated necrosis in the brain and heart by D-gal. It significantly reduced brain caspase 3, calbindin, and calcium-binding adapter molecule 1 (IBA1), heart caspase 3, and BCL2. Expression of mRNA of the brain and heart TP53, p21, Bax, and CASP-3 were significantly downregulated in the TQ and Cur combination group along with upregulation of BCL2 in comparison with the D-gal group. Data suggested that the TQ and Cur combination is a promising approach in aging prevention.MDPIJournal Articlehttp://hdl.handle.net/2115/823851422-0067International Journal of Molecular Sciences221368392021-07-01enginfo:doi/10.3390/ijms22136839none