2024-03-28T14:13:04Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/826652022-11-17T02:08:08Zhdl_2115_20055hdl_2115_8527Mevalonate Pathway-mediated ER Homeostasis Is Required for Haploid Stability in Human Somatic CellsYaguchi, KanSato, KiminoYoshizawa, KoyaMikami, DaisukeYuyama, KoheiIgarashi, YasuyukiBanhegyi, GaborMargittai, EvaUehara, RyotahaploidER stressMevalonate pathway463The somatic haploidy is unstable in diplontic animals, but cellular processes determining haploid stability remain elusive. Here, we found that inhibition of mevalonate pathway by pitavastatin, a widely used cholesterol-lowering drug, drastically destabilized the haploid state in HAP1 cells. Interestingly, cholesterol supplementation did not restore haploid stability in pitavastatin-treated cells, and cholesterol inhibitor U18666A did not phenocopy haploid destabilization. These results ruled out the involvement of cholesterol in haploid stability. Besides cholesterol perturbation, pitavastatin induced endoplasmic reticulum (ER) stress, the suppression of which by a chemical chaperon significantly restored haploid stability in pitavastatin-treated cells. Our data demonstrate the involvement of the mevalonate pathway in the stability of the haploid state in human somatic cells through managing ER stress, highlighting a novel link between ploidy and ER homeostatic control.一般社団法人 日本細胞生物学会(Japan Society for Cell Biology)Journal Articlehttp://hdl.handle.net/2115/826650386-7196Cell structure and function461192021-09-14enginfo:doi/10.1247/csf.20055none