2024-03-29T08:15:11Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/833842022-11-17T02:08:08Zhdl_2115_35410hdl_2115_35409Elevated Myeloperoxidase-DNA Complex Levels in Sera of Patients with IgA VasculitisTakeuchi, SoraKawakami, TamihiroOkano, TatsuroShida, HarukiNakazawa, DaigoTomaru, UtanoIshizu, AkihiroKadono, TakafumiIgA vasculitisNeutrophil extracellular trapsAntineutrophil cytoplasmic antibodyRenal complications490Introduction: IgA vasculitis is a systemic disease that results from the entrapment of circulating IgA-containing immune complexes in small-vessel walls in the skin, kidneys, and gastrointestinal tract. An excessive formation of neutrophil extracellular traps (NETs) is involved in the pathogenesis of vasculitis, especially in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis. This study aimed to clarify whether NETs are implicated in IgA vasculitis. Methods: Twenty-two patients with IgA vasculitis and 4 healthy volunteers were enrolled in this study. Serum levels of myeloperoxidase (MPO)-DNA complex, a fragment derived from NETs, were determined by enzyme-linked immunosorbent assay (ELISA), and the association between MPO-DNA complex levels and clinical parameters was examined. The presence of the ANCA was also assessed by ELISA specific for MPO and proteinase 3 (PR3) and indirect immunofluorescence (IIF), followed by assessing the differences in clinical parameters with and without the ANCA. Results: Serum MPO-DNA complex levels were significantly higher in patients with IgA vasculitis than those in healthy controls. A significant positive correlation between the serum MPO-DNA complex and IgA levels was noted. Interestingly, 63.6% of IgA vasculitis patients were ANCA-positive in IIF with an atypical pattern, whereas neither MPO-ANCA nor PR3-ANCA was detected by ELISA. These findings indicated that some IgA vasculitis patients possessed the so called minor ANCA. Serum IgA and MPO-DNA complex levels and the frequency of hematuria in the minor ANCA-positive group were significantly higher than in the minor ANCA-negative group. Conclusion: The collective findings suggested that NETs are certainly involved in the pathogenesis of IgA vasculitis.KargerJournal Articleapplication/pdfhttp://hdl.handle.net/2115/83384https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/83384/1/Pathobiology_Takeuchi%20et%20al.pdf1015-20081423-0291Pathobiology89123282021-11-23enginfo:pmid/34814154info:doi/10.1159/000519869https://creativecommons.org/licenses/by/4.0/publisher