2024-03-28T22:15:33Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/864992022-11-17T02:08:08Zhdl_2115_35410hdl_2115_35409A mouse model of short-term, diet-induced fatty liver with abnormal cardiolipin remodeling via downregulated Tafazzin gene expressionSakurai, ToshihiroChen, ZhenYamahata, ArisaHayasaka, TakahiroSatoh, HiroshiSekiguchi, HirotakaChiba, HitoshiHui, Shu-Pinghigh-carbohydrate dietliquid chromatography mass spectrometrylipidomicsmitochondriamonolysocardiolipin490Background Cardiolipin (CL) helps maintain mitochondrial structure and function. Here we investigated whether a high carbohydrate diet (HCD) fed to mice for a short period (5 days) could modulate the CL level, including that of monolysoCL (MLCL) in the liver. Results Total CL in the HCD group was 22% lower than that in the normal chow diet (NCD) group (P < 0.05). The CL72:8 level strikingly decreased by 93% (P < 0.0001), whereas total nascent CLs (CLs other than CL72:8) increased (P < 0.01) in the HCD group. The total MLCL in the HCD group increased by 2.4-fold compared with that in the NCD group (P < 0.05). Tafazzin expression in the HCD group was significantly downregulated compared with that in the NCD group (P < 0.05). A strong positive correlation between nascent CL and total MLCL (r = 0.955, P < 0.0001), and a negative correlation between MLCL and Tafazzin expression (r = -0.593, P = 0.0883) were observed. Conclusion A HCD modulated the fatty acid composition of CL and MLCL via Tafazzin in the liver, which could lead to mitochondrial dysfunction. This model may be useful for elucidating the relationship between fatty liver and mitochondrial dysfunction. (c) 2021 Society of Chemical IndustryJohn Wiley & SonsJournal Articleapplication/pdfhttp://hdl.handle.net/2115/86499https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/86499/1/20210115_MLCL_Taz_Manuscript_Resubmission_Final%20ver.pdf0022-5142Journal of the science of food and agriculture10112499550012021-08-05enginfo:pmid/33543498info:doi/10.1002/jsfa.11144This is the peer reviewed version of the following article: Sakurai, T., Chen, Z., Yamahata, A., Hayasaka, T., Satoh, H., Sekiguchi, H., Chiba, H., & Hui, S. P. (2021). A mouse model of short-term, diet-induced fatty liver with abnormal cardiolipin remodeling via downregulated Tafazzin gene expression. Journal of the science of food and agriculture, 101(12), 4995-5001, which has been published in final form at https://doi.org/10.1002/jsfa.11144. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions. This article may not be enhanced, enriched or otherwise transformed into a derivative work, without express permission from Wiley or by statutory rights under applicable legislation. Copyright notices must not be removed, obscured or modified. The article must be linked to Wiley’s version of record on Wiley Online Library and any embedding, framing or otherwise making available the article or pages thereof by third parties from platforms, services and websites other than Wiley Online Library must be prohibited.author