2024-03-28T20:56:51Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/866662022-11-17T02:08:08Zhdl_2115_20048hdl_2115_140Clarifying expression patterns by renal lesion using transcriptome analysis and vanin-1 as a potential novel biomarker for renal injury in chickensIshii, ChihiroKawai, Yusuke K.Ikenaka, YoshinoriMaekawa, NaoyaIchii, OsamuNakayama, Shouta M. M.Ishizuka, Mayumiacute interstitial nephritis (AIN)acute tubular necrosis (ATN)birdVNN1 (vanin-1)renal biomarker649Bird death is often caused by renal lesions induced by chemicals. The avian kidney has a renal portal system with significant blood flow that is sensitive to many chemicals. However, early avian bio-markers for kidney injury are yet to be identified. This study aimed to identify novel renal biomarkers. Acute kidney injury (AKI) can be divided into acute intersti-tial nephritis (AIN) and acute tubular necrosis (ATN). A chicken model of kidney damage was created by an injection of diclofenac or cisplatin, which caused either AIN or ATN, respectively. Microarray analysis was per-formed to profile the gene expression patterns in the chickens with nephropathy. A gene enrichment analysis suggested that the genes related to responses to external stimuli showed expression changes in both AIN and ATN. However, hierarchical clustering analyses sug-gested that gene expression patterns differed between AIN and ATN, and the number of biomarkers relating to renal damage was low. To identify early biomarkers for nephropathy, we focused on genes that were induced at various levels of renal damage. The gene, vanin-1 (VNN1) was highly induced in the early stages of renal damage. A quantitative real-time PCR analysis sup-ported this finding. These results suggest VNN1 could be a useful early biomarker of kidney injury in avian species.ElsevierJournal Articlehttp://hdl.handle.net/2115/866660032-5791Poultry Science10191020112022-09enginfo:doi/10.1016/j.psj.2022.102011none