2024-03-29T06:57:13Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/885792023-03-24T01:00:03Zhdl_2115_20045hdl_2115_139Interfacial biosilica coating of chitosan gel using fusion silicatein to fabricate robust hybrid material for biomolecular applicationsGodigamuwa, KasunNakashima, KazunoriTsujitani, SotaNaota, RyoMaulidin, IlhamKawasaki, Satoru519An enzyme-encapsulated silica-based hybrid material was developed using a chitosan gel. Fusion silicatein (InaKC-ChBD-Sil), silicatein fused with a soluble tag and chitin-binding domain, was employed as an interfacial catalyst to form silica on a chitosan gel matrix under physiological conditions, and horseradish peroxidase was immobilised on the hybrid material. Silica formation on the gel was verified via fluorescence microscopy using a designed fusion protein called TBP-mCherry, a fluorescent protein fused with a silica-binding peptide. We report a chitosan gel-silica hybrid material capable of encapsulating enzymes for biomedical and environmental applications.Royal Society of ChemistryJournal Articlehttp://hdl.handle.net/2115/885792050-750XJournal of materials chemistry B118165416582023-02-28enginfo:doi/10.1039/d2tb02581gnone