2024-03-29T04:43:49Zhttps://eprints.lib.hokudai.ac.jp/dspace-oai/requestoai:eprints.lib.hokudai.ac.jp:2115/9082022-11-17T02:08:08Zhdl_2115_20046hdl_2115_138Glucose and ecdysteroid increase apyrene sperm production in in vitro cultivation of spermatocysts of Bombyx moriKawamura, N.Sahara, K.Fugo, H.SilkwormIn vitro cultureEupyrene and apyrene sperm20-HydroxyecdysoneGlucose633Two types of sperm, nucleate eupyrene and anucleate apyrene, occur in the silkworm as in other lepidopteran species. Hormones and other substances have been assumed to play important roles in sperm dimorphism. We established an in vitro cultivation system for silkworm spermatocytes, and found that apyrene sperm are not produced when spermatocytes from larval testes are cultivated, though eupyrene spermatocytes develop normally into mature sperm. Based on the fact that ecdysteroid titers increase rapidly and peak 1 day after spinning, and that the amount of glycogen reaches its peak 1 day before the spinning stage, we studied the effects of adding glucose and/or 20-hydroxyecdysone to the culture medium. The experiments disclosed a significant additive effect of both substances on apyrene sperm production.Elsevier Science Ltd.Journal Articleapplication/pdfhttp://hdl.handle.net/2115/908https://eprints.lib.hokudai.ac.jp/dspace/bitstream/2115/908/1/JIP49%281%29.pdf0022-1910Journal of Insect Physiology49125302003-02-08enginfo:doi/10.1016/S0022-1910(02)00223-8author