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http://hdl.handle.net/2115/140
2024-03-29T09:59:02ZEvaluation of the biofilm detection capacity of the Congo Red Agar method for bovine mastitis-causing bacteria
http://hdl.handle.net/2115/91316
Title: Evaluation of the biofilm detection capacity of the Congo Red Agar method for bovine mastitis-causing bacteria
Authors: Mori, Shunsuke; Yamada, Atsumi; Kawai, Kazuhiro2024-02-26T15:00:00ZMori, ShunsukeYamada, AtsumiKawai, KazuhiroDetermination of Leptospiral antigens in naturally infected canine uterus by immunohistochemical immunofluorescence and ELISA methods
http://hdl.handle.net/2115/91312
Title: Determination of Leptospiral antigens in naturally infected canine uterus by immunohistochemical immunofluorescence and ELISA methods
Authors: Bolat, Ismail; Saglam, Yavuz Selim; Cengiz, Seyda; Yildirim, Serkan2024-02-26T15:00:00ZBolat, IsmailSaglam, Yavuz SelimCengiz, SeydaYildirim, SerkanInvestigation of the subchronic effects of low-dose pesticide mixture on rat testes
http://hdl.handle.net/2115/91306
Title: Investigation of the subchronic effects of low-dose pesticide mixture on rat testes
Authors: Ömür, Ali Doğan; Sevim, Çiğdem; Sağlam, Yavuz Selim; Bolat, İsmail; Genç, Sıdıka; Yeni, Yeşim; Aydın, Mehmet Akif; Sunar, Serhat; Koçak, Gökhan; Akarsu, Serkan Ali2024-02-26T15:00:00ZÖmür, Ali DoğanSevim, ÇiğdemSağlam, Yavuz SelimBolat, İsmailGenç, SıdıkaYeni, YeşimAydın, Mehmet AkifSunar, SerhatKoçak, GökhanAkarsu, Serkan AliThe value of evaluating luteal blood flow on the day of embryo transfer for recipient selection in Holstein lactating dairy cows
http://hdl.handle.net/2115/91304
Title: The value of evaluating luteal blood flow on the day of embryo transfer for recipient selection in Holstein lactating dairy cows
Authors: Tanida, Takashi; Mukono, Itsuro; Nakahashi, Mikiko; Miyano, Daiki; Miyazawa, Kurumi; Yanagawa, Yojiro; Katagiri, Seiji2024-02-26T15:00:00ZTanida, TakashiMukono, ItsuroNakahashi, MikikoMiyano, DaikiMiyazawa, KurumiYanagawa, YojiroKatagiri, SeijiSulfotransferases (SULTs), enzymatic and genetic variation in Carnivora : Limited sulfation capacity in pinnipeds
http://hdl.handle.net/2115/91043
Title: Sulfotransferases (SULTs), enzymatic and genetic variation in Carnivora : Limited sulfation capacity in pinnipeds
Authors: Kondo, Mitsuki; Ikenaka, Yoshinori; Nakayama, Shouta M. M.; Kawai, Yusuke K.; Mizukawa, Hazuki; Mitani, Yoko; Nomyama, Kei; Tanabe, Shinsuke; Ishizuka, Mayumi
Abstract: Wild carnivorans are one of the most important species due to their high positions in the food chain. They are also highly affected by numerous environmental contaminants through bioaccumulation and biomagnification. Xenobiotic metabolism is a significant chemical defense system from xenobiotics because it degrades the activity of a wide range of chemicals, generally into less active forms, resulting in their deactivation. Sulfotransferases (SULTs) are one of the most important xenobiotic metabolic enzymes, which catalyze the sulfonation of a variety of endogenous and exogenous chemicals, such as hormones, neurotransmitters, and a wide range of xenobiotic compounds. Although SULTs are of such high importance, little research has focused on these enzymes in wild carnivorans. In this study, we clarified the genetic properties of SULTs in a wide range of mammals, focusing on carnivorans, using in silico genetic analyses. We found genetic deficiencies of SULT1E1 and SULT1D1 isoforms in all pinnipeds analyzed and nonsense mutations in SULT1Cs in several carnivorans including pinnipeds. We further investigated the enzymatic activity of SULT1E1 in vitro using liver cytosols from pinnipeds. Using a SULT1E1 probe substrate, we found highly limited estradiol sulfonation in pinnipeds, whereas other mammals had relatively high sulfation. These results suggest that pinnipeds have severely or completely absent SULT1E1 activity, which importantly catalyzes the metabolism of estrogens, drugs, and environmental toxins. This further implies a high susceptibility to a wide range of xenobiotics in these carnivorans, which are constantly exposed to environmental chemicals throughout their lifetime.2022-12-31T15:00:00ZKondo, MitsukiIkenaka, YoshinoriNakayama, Shouta M. M.Kawai, Yusuke K.Mizukawa, HazukiMitani, YokoNomyama, KeiTanabe, ShinsukeIshizuka, MayumiWild carnivorans are one of the most important species due to their high positions in the food chain. They are also highly affected by numerous environmental contaminants through bioaccumulation and biomagnification. Xenobiotic metabolism is a significant chemical defense system from xenobiotics because it degrades the activity of a wide range of chemicals, generally into less active forms, resulting in their deactivation. Sulfotransferases (SULTs) are one of the most important xenobiotic metabolic enzymes, which catalyze the sulfonation of a variety of endogenous and exogenous chemicals, such as hormones, neurotransmitters, and a wide range of xenobiotic compounds. Although SULTs are of such high importance, little research has focused on these enzymes in wild carnivorans. In this study, we clarified the genetic properties of SULTs in a wide range of mammals, focusing on carnivorans, using in silico genetic analyses. We found genetic deficiencies of SULT1E1 and SULT1D1 isoforms in all pinnipeds analyzed and nonsense mutations in SULT1Cs in several carnivorans including pinnipeds. We further investigated the enzymatic activity of SULT1E1 in vitro using liver cytosols from pinnipeds. Using a SULT1E1 probe substrate, we found highly limited estradiol sulfonation in pinnipeds, whereas other mammals had relatively high sulfation. These results suggest that pinnipeds have severely or completely absent SULT1E1 activity, which importantly catalyzes the metabolism of estrogens, drugs, and environmental toxins. This further implies a high susceptibility to a wide range of xenobiotics in these carnivorans, which are constantly exposed to environmental chemicals throughout their lifetime.Expression of Indian hedgehog signaling in murine oviductal infundibulum and its relationship with epithelial homeostasis
http://hdl.handle.net/2115/91042
Title: Expression of Indian hedgehog signaling in murine oviductal infundibulum and its relationship with epithelial homeostasis
Authors: Hosotani, Marina; Ichii, Osamu; Namba, Takashi; Masum, Md Abdul; Nakamura, Teppei; Hasegawa, Yasuhiro; Watanabe, Takafumi; Kon, Yasuhiro
Abstract: Homeostasis of the oviductal infundibulum epithelium is continuously regulated by signaling pathways under physiological and pathological conditions. Herein, we investigated the expression of hedgehog (Hh) signaling-related components in the murine oviductal infundibulum, which is known to maintain homeostasis in the adult epithelium. Additionally, using autoimmune disease-prone MRL/MpJ-Fas(lpr/lpr) (MRL/lpr) mice showing abnormal morphofunction of the ciliated epithelium of the infundibulum related to the oviductal inflammation, we examined the relationship between Hh signaling and pathology of the infundibulum. The expression and localization of Pax8, a marker for progenitor cells in the oviductal epithelium, and Foxj1, a marker for ciliogenesis, were examined in the infundibulum. The results showed that Pax8 was downregulated and Foxj1 was upregulated with aging, suggesting that homeostasis of the infundibulum epithelium of MRL/lpr mice was disturbed at 6 months of age. In all mice, the motile cilia of ciliated epithelial cells in the infundibulum harbored Hh signaling pathway-related molecules: patched (Ptch), smoothened (Smo), and epithelial cells harbor Gli. In contrast, Ptch, Smo, and Gli2 were significantly downregulated in the infundibulum of MRL/lpr mice at 6 months of age. The expression levels of Pax8 and Foxj1 were significantly positively correlated with those of Ptch1, Smo, and Gli2. Hh signaling is thought to be involved in homeostasis of the ciliated epithelium in the infundibulum. In MRL/lpr mice, which show exacerbated severe systemic autoimmune abnormalities, molecular alterations in Hh signaling-related components are considered to interact with local inflammation in the infundibulum, leading to disturbances in epithelial homeostasis and reproductive function.2022-12-28T15:00:00ZHosotani, MarinaIchii, OsamuNamba, TakashiMasum, Md AbdulNakamura, TeppeiHasegawa, YasuhiroWatanabe, TakafumiKon, YasuhiroHomeostasis of the oviductal infundibulum epithelium is continuously regulated by signaling pathways under physiological and pathological conditions. Herein, we investigated the expression of hedgehog (Hh) signaling-related components in the murine oviductal infundibulum, which is known to maintain homeostasis in the adult epithelium. Additionally, using autoimmune disease-prone MRL/MpJ-Fas(lpr/lpr) (MRL/lpr) mice showing abnormal morphofunction of the ciliated epithelium of the infundibulum related to the oviductal inflammation, we examined the relationship between Hh signaling and pathology of the infundibulum. The expression and localization of Pax8, a marker for progenitor cells in the oviductal epithelium, and Foxj1, a marker for ciliogenesis, were examined in the infundibulum. The results showed that Pax8 was downregulated and Foxj1 was upregulated with aging, suggesting that homeostasis of the infundibulum epithelium of MRL/lpr mice was disturbed at 6 months of age. In all mice, the motile cilia of ciliated epithelial cells in the infundibulum harbored Hh signaling pathway-related molecules: patched (Ptch), smoothened (Smo), and epithelial cells harbor Gli. In contrast, Ptch, Smo, and Gli2 were significantly downregulated in the infundibulum of MRL/lpr mice at 6 months of age. The expression levels of Pax8 and Foxj1 were significantly positively correlated with those of Ptch1, Smo, and Gli2. Hh signaling is thought to be involved in homeostasis of the ciliated epithelium in the infundibulum. In MRL/lpr mice, which show exacerbated severe systemic autoimmune abnormalities, molecular alterations in Hh signaling-related components are considered to interact with local inflammation in the infundibulum, leading to disturbances in epithelial homeostasis and reproductive function.Modified foreign body reaction to silicone imbedded in subcutaneous tissues by different mouse systemic immune conditions
http://hdl.handle.net/2115/91025
Title: Modified foreign body reaction to silicone imbedded in subcutaneous tissues by different mouse systemic immune conditions
Authors: Yamakawa, Tomohiro; Ichii, Osamu; Nakamura, Teppei; Namba, Takashi; Elewa, Yaser Hosny Ali; Masum, Md. Abdul; Otani, Yuki; Nishimura, Takanori; Kon, Yasuhiro
Abstract: Foreign body reaction (FBR) causes unexpected adverse effects due to implanted materials in humans and animals. Inflammation and subsequent fibrosis during FBR seems to be affected by recipient immunity, such as the balance of T helper (Th) response that has the potential to regulate FBR-related macrophage function. Here, the immunological effects of FBR on subcutaneously imbedded silicone tubes (ST) at 8 weeks were investigated histologically by comparing Th1-biased C57BL/6N, Th2-biased MRL/MpJ, and autoimmune disease-prone MRL/MpJ-Fas(lpr/lpr). Tissue surrounding ST (TSS) was analyzed at day (D) 7 and 14 (reaction phase) or D35 (stability phase) after surgery. In all strains, the TSS was composed of a thin layer (TL) containing fibrous tissues and loose connective tissues formed outside the TL. Few lymphocytes and mast cells, several neutrophils, and numerous macrophages infiltrated the TSS. Active vascularization was observed at D14 in all strains. For the examined indices, M1-type macrophage density in the TSS of C57BL/6N mice was significantly higher at D14 compared to other strains. No significant strain difference relating to M2-type macrophages was detected, suggesting the effects of Th1-biased immunity on FBR-related inflammation. Collagen fibers in the TSS increased in density and became stable with age in all strains. In particular, MRL/MpJ-Fas(lpr/lpr) showed progressive fibrotic features. Serum autoantibody levels in MRL/MpJ-Fas(lpr/lpr) mice were inversely correlated with M1-type macrophage density. These data from MRL/MpJ-Fas(lpr/lpr) mice suggested modifications of FBR-related inflammation and fibrosis by autoimmune abnormalities. The results provide crucial insights into the pathological modification of FBR by recipient immunity and emphasize its clinicopathological importance in humans and animals.2022-06-29T15:00:00ZYamakawa, TomohiroIchii, OsamuNakamura, TeppeiNamba, TakashiElewa, Yaser Hosny AliMasum, Md. AbdulOtani, YukiNishimura, TakanoriKon, YasuhiroForeign body reaction (FBR) causes unexpected adverse effects due to implanted materials in humans and animals. Inflammation and subsequent fibrosis during FBR seems to be affected by recipient immunity, such as the balance of T helper (Th) response that has the potential to regulate FBR-related macrophage function. Here, the immunological effects of FBR on subcutaneously imbedded silicone tubes (ST) at 8 weeks were investigated histologically by comparing Th1-biased C57BL/6N, Th2-biased MRL/MpJ, and autoimmune disease-prone MRL/MpJ-Fas(lpr/lpr). Tissue surrounding ST (TSS) was analyzed at day (D) 7 and 14 (reaction phase) or D35 (stability phase) after surgery. In all strains, the TSS was composed of a thin layer (TL) containing fibrous tissues and loose connective tissues formed outside the TL. Few lymphocytes and mast cells, several neutrophils, and numerous macrophages infiltrated the TSS. Active vascularization was observed at D14 in all strains. For the examined indices, M1-type macrophage density in the TSS of C57BL/6N mice was significantly higher at D14 compared to other strains. No significant strain difference relating to M2-type macrophages was detected, suggesting the effects of Th1-biased immunity on FBR-related inflammation. Collagen fibers in the TSS increased in density and became stable with age in all strains. In particular, MRL/MpJ-Fas(lpr/lpr) showed progressive fibrotic features. Serum autoantibody levels in MRL/MpJ-Fas(lpr/lpr) mice were inversely correlated with M1-type macrophage density. These data from MRL/MpJ-Fas(lpr/lpr) mice suggested modifications of FBR-related inflammation and fibrosis by autoimmune abnormalities. The results provide crucial insights into the pathological modification of FBR by recipient immunity and emphasize its clinicopathological importance in humans and animals.Ureteral morphology and pathology during urolithiasis in cats
http://hdl.handle.net/2115/90938
Title: Ureteral morphology and pathology during urolithiasis in cats
Authors: Ichii, Osamu; Oyamada, Kazuhisa; Mizukawa, Hazuki; Yokoyama, Nozomu; Namba, Takashi; Otani, Yuki; Elewa, Yaser Hosny Ali; Sasaki, Noboru; Nakamura, Teppei; Kon, Yasuhiro
Abstract: Cats exhibit high susceptibility to urinary organ-related diseases. We investigated the healthy ureter morphol-ogies and compared these with ureters that were surgically resected distal to a urolithiasis obstruction in cats. Healthy ureters (total length 9.88 +/- 0.38 cm) developed adventitia composed of collagen fibers (ADCF), con-taining a longitudinal muscular layer, toward the distal segment. The healthy ureter was the smallest in the middle segment (4.71-6.90 cm from the urinary bladder) with significantly decreased luminal and submucosal areas compared to those in the proximal segment. Diseased cats exhibited a high incidence of calcium oxalate urolithiasis with renal dysfunction, regardless of age, sex, and body size. Diseased ureters showed increased perimeters, inflammation, and decreased nerves in ADCF. Collagen fibers were increased in the submucosal area, intermuscular spaces, and ADCF, particularly near the obstructed lesion. The mean resected ureter length was 5.66 +/- 0.49 cm, suggesting a high obstruction risk in the middle segment. The middle segment also increased the cross-sectional area of the ureter and ADCF, regardless of the distance from the obstructed lesion. The ureters in several cases either lacked the transitional epithelium, or exhibited transitional epithelial hyperplasia, and some of these formed the mucosal folds. In conclusion, we demonstrated the following characteristics and histo-pathological features of cat ureters: decreases in the ureter size, lumen area, and submucosa area from proximal to middle segment in healthy; ADCF changes in urolithiasis, including increased connective tissues with inflammation and decreased nerves. These data are important to understand the pathogenesis of feline ureteral obstruction.2022-12-09T15:00:00ZIchii, OsamuOyamada, KazuhisaMizukawa, HazukiYokoyama, NozomuNamba, TakashiOtani, YukiElewa, Yaser Hosny AliSasaki, NoboruNakamura, TeppeiKon, YasuhiroCats exhibit high susceptibility to urinary organ-related diseases. We investigated the healthy ureter morphol-ogies and compared these with ureters that were surgically resected distal to a urolithiasis obstruction in cats. Healthy ureters (total length 9.88 +/- 0.38 cm) developed adventitia composed of collagen fibers (ADCF), con-taining a longitudinal muscular layer, toward the distal segment. The healthy ureter was the smallest in the middle segment (4.71-6.90 cm from the urinary bladder) with significantly decreased luminal and submucosal areas compared to those in the proximal segment. Diseased cats exhibited a high incidence of calcium oxalate urolithiasis with renal dysfunction, regardless of age, sex, and body size. Diseased ureters showed increased perimeters, inflammation, and decreased nerves in ADCF. Collagen fibers were increased in the submucosal area, intermuscular spaces, and ADCF, particularly near the obstructed lesion. The mean resected ureter length was 5.66 +/- 0.49 cm, suggesting a high obstruction risk in the middle segment. The middle segment also increased the cross-sectional area of the ureter and ADCF, regardless of the distance from the obstructed lesion. The ureters in several cases either lacked the transitional epithelium, or exhibited transitional epithelial hyperplasia, and some of these formed the mucosal folds. In conclusion, we demonstrated the following characteristics and histo-pathological features of cat ureters: decreases in the ureter size, lumen area, and submucosa area from proximal to middle segment in healthy; ADCF changes in urolithiasis, including increased connective tissues with inflammation and decreased nerves. These data are important to understand the pathogenesis of feline ureteral obstruction.Measurement of serum procalcitonin concentrations in calves with bovine respiratory disease
http://hdl.handle.net/2115/90821
Title: Measurement of serum procalcitonin concentrations in calves with bovine respiratory disease
Authors: Koshiishi, Tomoko; Shibutani, Shusaku; Chuma, Takehisa; Iwata, Hiroyuki2023-11-26T15:00:00ZKoshiishi, TomokoShibutani, ShusakuChuma, TakehisaIwata, HiroyukiCampylobacter contamination of chicken meat in Japan : geographical and seasonal variations
http://hdl.handle.net/2115/90820
Title: Campylobacter contamination of chicken meat in Japan : geographical and seasonal variations
Authors: Sasaki, Yoshimasa; Ikeda, Tetsuya; Yonemitsu, Kenzo; Okamura, Masashi; Uema, Masashi2023-11-26T15:00:00ZSasaki, YoshimasaIkeda, TetsuyaYonemitsu, KenzoOkamura, MasashiUema, Masashi