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New fluorometric enzyme immunoassay for 17β-estradiol by homogeneous reaction using biotinylated estradiol

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Title: New fluorometric enzyme immunoassay for 17β-estradiol by homogeneous reaction using biotinylated estradiol
Authors: Matsumoto, Yuko Browse this author
Kuramitz, Hideki Browse this author
Itoh, Shinji Browse this author
Tanaka, Shunitz Browse this author →KAKEN DB
Keywords: Fluorometric enzyme immunoassay
17β-Estradiol
Biotin-immobilized microtiter plate
Solid-phase avidin–biotin binding assay
Issue Date: 15-May-2006
Publisher: Elsevier
Journal Title: Talanta
Volume: 69
Issue: 3
Start Page: 663
End Page: 668
Publisher DOI: 10.1016/j.talanta.2005.10.040
PMID: 18970619
Abstract: A new fluorometric enzyme immunoassay for 17β-estradiol (E2) using biotinylated estradiol (BE) as a probe ligand, is described. In this method, E2 is detected indirectly by a solid-phase avidin-biotin binding assay, in which the biotin is immobilized on a microtiter plate (biotin-plate). After the competitive reaction between E2 and BE for the anti-E2 antibody in solution, the free E2 and BE are separated from the bound forms by means of ultrafiltration. The concentration of BE in the solution is determined from the reaction between the biotin immobilized on the plate and the free BE for the limited biotin binding sites of avidin conjugated with horseradish peroxidase (avidin-HRP), which is added to the solution. The enzymatic reaction of HRP was TM measured by a fluorometric analysis with the QuantaBlu Fluorogenic Peroxidase Substrate (QFPS) in order to detect of the avidin-biotin binding with a high degree of sensitivity. The detection limit and linear range for the determination of E2 were 0.12 nM and from 0.12 to 25 nM, respectively. The relative standard deviations (RSD) for the E2 assay were between 2.2 and 9.1% (n = 3). The cross-reactivity for several other estrogens was also evaluated.
Relation: http://www.sciencedirect.com/science/journal/00399140
Type: article (author version)
URI: http://hdl.handle.net/2115/14472
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 松本 優子

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