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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Expression of Bovine Lactoferrin C-lobe in Rhodococcus erythropolis and Its Purification and Characterization
| Title: | Expression of Bovine Lactoferrin C-lobe in Rhodococcus erythropolis and Its Purification and Characterization |
| Authors: | Kim, Woan-Sub Browse this author | | Shimazaki, Kei-ichi Browse this author | | Tamura, Tomohiro Browse this author →KAKEN DB |
| Keywords: | lactoferrin | | Rhodococcus | | gene expression | | antibacterial activity | | milk |
| Issue Date: | Nov-2006 |
| Publisher: | Japan Society for Bioscience, Biotechnology, and Agrochemistry |
| Journal Title: | Bioscience Biotechnology and Biochemistry |
| Volume: | 70 |
| Issue: | 11 |
| Start Page: | 2641 |
| End Page: | 2645 |
| Publisher DOI: | 10.1271/bbb.60245 |
| PMID: | 17090917 |
| Abstract: | A Rhodococcus erythropolis expression system for the bovine lactoferrin C-lobe was constructed. The DNA fragments encoding the BLF C-lobe were amplified and cloned into vector pTip LCH1.2. R. erythropolis carrying the pTip-C-lobe was cultured at 30 °C with shaking, and expression of the rBLF C-lobe was induced by adding 1 μg/ml (final concentration) thiostrepton. The rBLF C-lobe was isolated in native and denatured (8 M urea) form by Ni-NTA affinity chromatography. To obtain a bioactive rBLF C-lobe, the protein isolated in the denatured form was refolded by stepwise dialysis against refolding buffers. The antibacterial activity of the rBLF C-lobe was tested by the filter-disc plate assay method. The refolded rBLF C-lobe demonstrated antibacterial activity against selected strains of Escherichia coli. |
| Relation: | http://www.jstage.jst.go.jp/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/17011 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 田村 具博
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