HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine >
Japanese Journal of Veterinary Research >
Volume 16, Number 1 >

IMMUNOFLUORESCENCE AND CELLULAR FRACTION STUDIES ON THE MULTIPLICATION OF INFECTIOUS CANINE HEPATITIS VIRUS

Files in This Item:
KJ00002369707.pdf629.75 kBPDFView/Open
Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.16.1.13

Title: IMMUNOFLUORESCENCE AND CELLULAR FRACTION STUDIES ON THE MULTIPLICATION OF INFECTIOUS CANINE HEPATITIS VIRUS
Authors: SUGIMURA, Takaaki Browse this author
YANAGAWA, Ryo Browse this author
Issue Date: Mar-1968
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 16
Issue: 1
Start Page: 13
End Page: 21
Abstract: The process of multiplication of ICH virus in the nucleus and cytoplasm of the infected dog kidney cell cultures were studied by cellular fractionation and immunofluorescence techniques. The infectivity was detected in the nuclear fraction 12 hrs after infection. The virus titer was always higher in the nuclear fraction than in the cytoplasmic fraction throughout the course of infection. CF and HA titers were detected in the cytoplasmic fraction 16 hrs after infection, while in the nuclear fraction both were detected after 24 hrs. The titers of these antigens were always found to be higher in the cytoplasmic fraction than in the nuclear fraction. Applying the fluorescent antibody technique using anti-CF antigen and anti-HA antigen sera, specific fluorescence was detected 9 hrs after infection. The CF antigen first appeared in the nucleus as small granules with less intense fluorescence. In the next stage, the fluorescence was found as a fibrous structure. The HA antigen was found throughout the nucleus as granules with bright fluorescence, and never found as a fibrous structure. When the infectious process advanced, no difference was found between the infected cells stained with anti-CF and -HA antigen sera.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/1894
Appears in Collections:Japanese Journal of Veterinary Research > Volume 16, Number 1

Submitter: 獣医学部図書室

Export metadata:

OAI-PMH ( junii2 , jpcoar )

MathJax is now OFF:


 

Feedback - Hokkaido University