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Japanese Journal of Veterinary Research >
Volume 21, Number 3 >

ELECTROPHORETIC AND IMMUNOLOGICAL STUDIES ON THE LIGHT CHAINS OF MYOSIN

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.21.3.64

タイトル: ELECTROPHORETIC AND IMMUNOLOGICAL STUDIES ON THE LIGHT CHAINS OF MYOSIN
著者: KATOH, Norio 著作を一覧する
KUBO, Shuichiro 著作を一覧する
TAKAHASHI, Hiroshi 著作を一覧する
発行日: 1973年 7月
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 21
号: 3
開始ページ: 64
終了ページ: 72
抄録: Myosin was highly purified mainly by combining procedures of ammonium sulfate fractionation and chromatographies on cellulose phosphate, DEAE-cellulose and DEAE-Sephadex A-50 as judged by gel electrophoresis and immunological analyses. The highly purified myosin produced two precipitin lines in immunodiffusion with antibodies to myosin, whereas the myosin produced only one peak in quantitative precipitin analysis. This difference implied the possibility that agar gel promoted the dissociation of light chains from myosin. Immunological analyses of light chains prepared from the myosin showed the presence of two kinds of light chain components. On the other hand, light chains were separated usually into two components, L-1 (molecular weight 27,000) and L-2 (mol. wt. 17,000) in SDS-gel electrophoresis. However, an additional component, L-3 (mol. wt. 14,000) was sometimes appeared in the electrophoresis of light chains. The possibility that L-3 was a breakdown product of L-1 was discussed.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/2019
出現コレクション:Volume 21, Number 3

提供者: 獣医学部図書室

 

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