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Volume 21, Number 3 >

ELECTROPHORETIC AND IMMUNOLOGICAL STUDIES ON THE LIGHT CHAINS OF MYOSIN

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.21.3.64

Title: ELECTROPHORETIC AND IMMUNOLOGICAL STUDIES ON THE LIGHT CHAINS OF MYOSIN
Authors: KATOH, Norio Browse this author
KUBO, Shuichiro Browse this author
TAKAHASHI, Hiroshi Browse this author
Issue Date: Jul-1973
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 21
Issue: 3
Start Page: 64
End Page: 72
Abstract: Myosin was highly purified mainly by combining procedures of ammonium sulfate fractionation and chromatographies on cellulose phosphate, DEAE-cellulose and DEAE-Sephadex A-50 as judged by gel electrophoresis and immunological analyses. The highly purified myosin produced two precipitin lines in immunodiffusion with antibodies to myosin, whereas the myosin produced only one peak in quantitative precipitin analysis. This difference implied the possibility that agar gel promoted the dissociation of light chains from myosin. Immunological analyses of light chains prepared from the myosin showed the presence of two kinds of light chain components. On the other hand, light chains were separated usually into two components, L-1 (molecular weight 27,000) and L-2 (mol. wt. 17,000) in SDS-gel electrophoresis. However, an additional component, L-3 (mol. wt. 14,000) was sometimes appeared in the electrophoresis of light chains. The possibility that L-3 was a breakdown product of L-1 was discussed.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2019
Appears in Collections:Japanese Journal of Veterinary Research > Volume 21, Number 3

Submitter: 獣医学部図書室

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