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Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/20471

Title: Effects of Overexpression and Antisense RNA Expression of Orf17, a MutT-Type Enzyme
Authors: Hori, Mika Browse this author
Asanuma, Taketoshi Browse this author
Inanami, Osamu Browse this author →KAKEN DB
Kuwabara, Mikinori Browse this author
Harashima, Hideyoshi Browse this author →KAKEN DB
Kamiya, Hiroyuki Browse this author
Keywords: Orf17
MutT
8-hydroxy-dGTP
nucleotide pool sanitization
antisense RNA
Issue Date: Jun-2006
Publisher: Pharmaceutical Society of Japan
Journal Title: Biological & Pharmaceutical Bulletin
Volume: 29
Issue: 6
Start Page: 1087
End Page: 1091
Publisher DOI: 10.1248/bpb.29.1087
Abstract: The Escherichia coli Orf17 (NtpA, NudB) protein, a MutT-type enzyme, hydrolyzes oxidized deoxyribonucleotides, including 8-hydroxy-2'-deoxyadenosine 5'-triphosphate and 8-hydroxy-2'-deoxyguanosine 5'-triphosphate, in vitro. To examine its in vivo role(s) in bacteria, plasmid DNAs containing the orf17 gene in the sense and antisense orientations were introduced. When the Orf17 protein was overexpressed in mutT cells, the rpoB mutant frequency was decreased. On the other hand, similar effects were not observed when Orf17 was overexpressed in wild type and orf135 cells. Expression of the antisense RNA of the orf17 gene did not produce an obvious phenotype, such as increased mutant frequency and resistance to ionizing radiation. These results suggest that the role of the Orf17 protein is to back up the MutT function, and to assist in the elimination of 8-hydroxy-2'-deoxyguanosine nucleotides.
Type: article
URI: http://hdl.handle.net/2115/20471
Appears in Collections:薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷 浩之

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