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Volume 43, Number 3-4  >

EFFECTS OF SUPPLEMENTATION OF THE MATURATION MEDIA WITH INSULIN ON IN VITRO MATURATION AND IN VITRO FERTILIZATION OF BOVINE OOCYTES

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Please use this identifier to cite or link to this item:https://doi.org/10.14943/jjvr.43.3-4.145

Title: EFFECTS OF SUPPLEMENTATION OF THE MATURATION MEDIA WITH INSULIN ON IN VITRO MATURATION AND IN VITRO FERTILIZATION OF BOVINE OOCYTES
Authors: MATSUI, Motozumi Browse this author
TAKAHASHI, Yoshiyuki Browse this author
HISHINUMA, Mitsugu Browse this author
KANAGAWA, Hiroshi Browse this author
Keywords: insulin
bovine oocyte
in vitro maturation
in vitro fertilization
Issue Date: 27-Dec-1995
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 43
Issue: 3-4
Start Page: 145
End Page: 153
Abstract: This study was carried out to determine the effects of supplementation of the maturation media with insulin on in vitro maturation and fertilization of bovine oocytes. In Experiment 1,cumulus-intact bovine oocytes were cultured in a maturation medium (TCM-199 containing 10% fetal calf serum, 0.02 U/ml follicular stimulating hormone and 1 μg/ml estradiol-17 β) with or without insulin supplementation (10 μg/ml). The maturation and fertilization rates of oocytes and subsequent embryonic development to the blastocyst stage were not affected by the treatment with insulin in the presence of serum and the hormones during the maturation period. In Experiment 2,to avoid the effects of serum and the hormones, a serum- and hormone-free maturation medium (TCM-199 containing 1 mg/ml polyvinyl alcohol) was used. In the absence of serum and hormones during the maturation period, the maturation rate was not affected by treatment with insulin, but the fertilization rate was improved. In Experiment 3,when denuded oocytes were inseminated together with cumulus cells cultured in serum- and hormone-free maturation medium supplemented with insulin, the fertilization rate was increased. These results demonstrate that the addition of insulin to the serum- and hormone-free maturation medium improves the fertilization rate of bovine oocytes in vitro, and suggest that insulin may stimulate the secretion of sperm capacitating agent (s) from cumulus cells.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2519
Appears in Collections:Japanese Journal of Veterinary Research > Volume 43, Number 3-4

Submitter: 獣医学部図書室

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