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A MONOCLONAL ANTIBODY, 169.1,AGAINST CANINE LEUKOCYTE SURFACE ANTIGEN IDENTIFIES CYTOSKELETAL COMPONENTS IN EPITHELIAL CELLS AND PERIPHERAL NEURONS

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Please use this identifier to cite or link to this item:https://doi.org/10.14943/jjvr.44.4.193

Title: A MONOCLONAL ANTIBODY, 169.1,AGAINST CANINE LEUKOCYTE SURFACE ANTIGEN IDENTIFIES CYTOSKELETAL COMPONENTS IN EPITHELIAL CELLS AND PERIPHERAL NEURONS
Authors: IWAMI, Yukihiko Browse this author
HASHIMOTO, Yoshiharu Browse this author →KAKEN DB
IWANAGA, Toshihiko Browse this author →KAKEN DB
Keywords: monoclonal antibody
canine leukocyte
flow cytometry
immunohistochemistry
cytoskeletal protein
Issue Date: 28-Feb-1997
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 44
Issue: 4
Start Page: 193
End Page: 206
Abstract: Monoclonal antibodies were produced by immunizing BALB/c mice with freshly prepared canine thymocytes and peripheral blood leukocytes. Flow cytometric analysis of canine peripheral blood leukocytes showed that an antibody, designated 169.1, recognized about 60% of non-lymphoid cells, but reacted with less than 3% of lymphocytes. Immunohistochemistry using frozen sections showed that several types of epithelial cells such as epithelial reticulum cells in the thymus and ductal system in the parotid gland exhibited an intense positive reaction to antibody 169.1. Moreover, the ellipsoidal sheath in the spleen and non-myelinated nerve fibers in the peripheral nervous system had selectively positive reactions ; in the latter, filamentous structures were visible under a light microscope. In contrast to the data from the flow cytometric analysis, no leukocytes on sections reacted with 169.1. Immunoblot assay revealed that 169.1 recognized antigens with molecular weights of 48 and 52 kDa under reducing conditions. These findings characterize 169.1 as an antibody against a cytoskeletal protein rich in epithelial cells and neurons.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2573
Appears in Collections:Japanese Journal of Veterinary Research > Volume 44, Number 4

Submitter: 獣医学部図書室

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