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Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription.
Title: | Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription. |
Authors: | Kamiya, Hiroyuki Browse this author | Suzuki, Akihiro Browse this author | Kawai, Kazuaki Browse this author | Kasai, Hiroshi Browse this author | Harashima, Hideyoshi Browse this author →KAKEN DB |
Keywords: | Ribonucleotide | 8-Hydroxy-GTP | 2-Hydroxy-ATP | Transcription |
Issue Date: | 1-Sep-2007 |
Publisher: | Elsevier |
Journal Title: | Free Radical Biology and Medicine |
Volume: | 43 |
Issue: | 5 |
Start Page: | 837 |
End Page: | 843 |
Publisher DOI: | 10.1016/j.freeradbiomed.2007.05.034 |
PMID: | 17664147 |
Abstract: | Oxidation of RNA precursors may disturb genetic information. In this study, the effects of oxidized RNA precursors on in vitro transcription were examined. Two oxidized ribonucleoside triphosphates, 8-hydroxyguanosine 5′-triphosphate (8-OH-GTP) and 2-hydroxyadenosine 5′-triphosphate (2-OH-ATP), were added to in vitro transcription reactions. The addition of 8-OH-GTP and 2-OH-ATP reduced the amount of RNA synthesized in vitro. Moreover, to examine qualitative alteration of the mRNA, it was converted to cDNA by reverse transcriptase, and the cDNA was then amplified by PCR. The PCR product was subsequently cloned into plasmid DNA, and the DNA sequence was analyzed for each bacterial colony. The two oxidized ribonucleotides induced mutations in cDNA, suggesting the disturbance of genetic information during transcription and/or reverse transcription. 8-OH-GTP induced T → G plus T → C mutations, and 2-OH-ATP caused T → C mutations. These results indicate that the formation of these oxidized RNA precursors in cells affects transcription quantitatively and qualitatively. |
Relation: | http://www.sciencedirect.com/science/journal/08915849 |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/27982 |
Appears in Collections: | 薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 紙谷 浩之
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