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Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein

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Title: Infectivity-Enhancing Antibodies to Ebola Virus Glycoprotein
Authors: Takada, Ayato Browse this author
Watanabe, Shinji Browse this author
Okazaki, Katsunori Browse this author
Kida, Hiroshi Browse this author
Kawaoka, Yoshihiro Browse this author
Issue Date: Mar-2001
Publisher: American Society for Microbiology
Journal Title: Journal of Virology
Volume: 75
Issue: 5
Start Page: 2324
End Page: 2330
Publisher DOI: 10.1128/JVI.75.5.2324-2330.2001
Abstract: Ebola virus causes severe hemorrhagic fever in primates, resulting in mortality rates of up to 100%, yet there are no satisfactory biologic explanations for this extreme virulence. Here we show that antisera produced by DNA immunization with a plasmid encoding the surface glycoprotein (GP) of the Zaire strain of Ebola virus enhances the infectivity of vesicular stomatitis virus pseudotyped with the GP. Substantially weaker enhancement was observed with antiserum to the GP of the Reston strain, which is much less pathogenic in humans than the Ebola Zaire and Sudan viruses. The enhancing activity was abolished by heat but was increased in the presence of complement system inhibitors, suggesting that heat-labile factors other than the complement system are required for this effect. We also generated an anti-Zaire GP monoclonal antibody that enhanced viral infectivity and another that neutralized it, indicating the presence of distinct epitopes for these properties. Our findings suggest that antibody-dependent enhancement of infectivity may account for the extreme virulence of the virus. They also raise issues about the development of Ebola virus vaccines and the use of passive prophylaxis or therapy with Ebola virus GP antibodies.
Rights: Copyright © 2001 American Society for Microbiology
Type: article
URI: http://hdl.handle.net/2115/28139
Appears in Collections:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 喜田 宏

 

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