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Volume 48, Number 4 >

A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.48.4.187

Title: A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR
Authors: OZAKI, Hiroichi Browse this author
SUGITA, Shigeo Browse this author
KIDA, Hiroshi Browse this author →KAKEN DB
Issue Date: 28-Feb-2001
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 48
Issue: 4
Start Page: 187
End Page: 195
Abstract: A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using imununo-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10^<1.0~2.0> times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10^<7.0~8.0> times higher than those by virus isolation or by RT-PCR.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/2859
Appears in Collections:Japanese Journal of Veterinary Research > Volume 48, Number 4

Submitter: 獣医学部図書室

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