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Japanese Journal of Veterinary Research >
Volume 48, Number 4 >

A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR

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この文献には次のDOIがあります:http://doi.org/10.14943/jjvr.48.4.187

タイトル: A Rapid and Highly Sensitive Method for Diagnosis of Equine Influenza by Antigen Detection Using Immuno-PCR
著者: OZAKI, Hiroichi 著作を一覧する
SUGITA, Shigeo 著作を一覧する
KIDA, Hiroshi 著作を一覧する
発行日: 2001年 2月28日
出版者: The Graduate School of Veterinary Medicine
誌名: Japanese Journal of Veterinary Research
巻: 48
号: 4
開始ページ: 187
終了ページ: 195
抄録: A rapid and highly sensitive method for diagnosis of influenza by detecting viral antigens using imununo-PCR has been developed. The sensitivity of the immuno-PCR for the detection of the nonstructural protein 1 (NS 1) antigenically common among influenza A viruses was 10^<1.0~2.0> times higher than that of RT-PCR for the detection of the viral genome. For the detection of the hemagglutinin (HA) subtype-specific antigen, this assay using anti-HA monoclonal antibodies attained a sensitivity of up to 10^<7.0~8.0> times higher than those by virus isolation or by RT-PCR.
資料タイプ: bulletin
URI: http://hdl.handle.net/2115/2859
出現コレクション:Volume 48, Number 4

 

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