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Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP

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タイトル: Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP
著者: Suzuki, Tetsuya 著作を一覧する
Yamamoto, Kazuo 著作を一覧する
Harashima, Hideyoshi 著作を一覧する
Kamiya, Hiroyuki 著作を一覧する
キーワード: Base excision repair
Nth
8-OH-dGTP
2-OH-dATP
発行日: 2008年 1月 1日
出版者: Elsevier
誌名: DNA Repair
巻: 7
号: 1
開始ページ: 88
終了ページ: 94
出版社 DOI: 10.1016/j.dnarep.2007.08.003
抄録: To examine whether base excision repair suppresses mutations induced by oxidized deoxyribonucleotide 5′-triphosphates in the nucleotide pool, 8-hydroxy-dGTP (8-OH-dGTP) and 2-hydroxy-dATP were introduced into Escherichia coli strains deficient in endonucleases III (Nth) and VIII (Nei) and MutY, and mutations in the chromosomal rpoB gene were analyzed. The spontaneous rpoB mutant frequency was also examined in mutT/nth and mutT/nei strains, to assess the influence on the mutations induced by the endogenous 8-OH-dGTP accumulated in the mutT mutant. The mutations induced by exogenous 2-hydroxy-dATP were similar in all of the strains tested. Exogenous 8-OH-dGTP increased the rpoB mutant frequency more efficiently in the nth strain than that in the wild-type strain. The spontaneous mutant frequency in the mutT/nth strain was 2-fold higher than that in the mutT strain. These results suggest that E. coli endonuclease III also acts as a defense against the mutations caused by 8-OH-dGTP in the nucleotide pool.
Relation (URI): http://www.sciencedirect.com/science/journal/15687864
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/32295
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 紙谷 浩之

 

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