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Volume 39, Number 1 >

THE NUCLEOTIDE SEQUENCE OF THE GENES, fanE AND fanF, OF Escherichia coli K99 FIMBRIAE

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.39.1.1

Title: THE NUCLEOTIDE SEQUENCE OF THE GENES, fanE AND fanF, OF Escherichia coli K99 FIMBRIAE
Authors: ONO, Eriko Browse this author
LAVIN, Martin F. Browse this author
NAIKI, Masaharu Browse this author
Keywords: Enterotoxigenic Escherichia coli
K99 fimbriae
fanE and fanF DNA sequence
hydrophobic domain
Issue Date: 30-May-1991
Publisher: The Graduate School of Veterinary Medicine
Journal Title: Japanese Journal of Veterinary Research
Volume: 39
Issue: 1
Start Page: 1
End Page: 10
Abstract: K99 fimbriae of enterotoxigenic Escherichia coli consist of eight different subunits. A major subunit called fimbrillin forms fimbrial structure and a minor subunit called adhesin localizes at the tip of fimbriae and recognizes host receptor ganglioside. Within this eight gene cluster, fanE and fanF have not yet been sequenced. In this study, fanE and fanF genes were sequenced by analyzing several DNA fragments produced by endonuclease or exonuclease digestion. The fanE gene encoded 227 amino acids containing 20 amino acids of signal peptide starting from GTG (valine) and showed a homology to fanA-fanB. The fanF gene encoded 271 amino acids containing 20 amino acids of signal peptide starting from ATG (methionine) and showed homologies to the fanD gene, fimbrillin gene of F41,adhesin gene of P fimbriae (papG) and adhesin gene of Type 1 fimbriae (fimH). E and F subunits had fifteen and fourteen hydrophobic domains, respectively, which periodically appeared possibly forming a hydrophobic region.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/3237
Appears in Collections:Japanese Journal of Veterinary Research > Volume 39, Number 1

Submitter: 獣医学部図書室

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