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Virucidal Effects of Ultraviolet, Heat Treatment and Disinfectants against Koi Herpesvirus (KHV)

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Title: Virucidal Effects of Ultraviolet, Heat Treatment and Disinfectants against Koi Herpesvirus (KHV)
Authors: Kasai, Hisae Browse this author →KAKEN DB
Muto, Yoshifumi Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Keywords: koi herpesvirus
KHV
virucidal effect
disinfectant
ultraviolet
Issue Date: 15-Sep-2005
Publisher: 日本魚病学会
Journal Title: 魚病研究
Journal Title(alt): Fish Pathology
Volume: 40
Issue: 3
Start Page: 137
End Page: 138
Abstract: The virucidal effects of ultraviolet (UV) irradiation, heat treatment and disinfectants against koi herpesvirus (KHV) were evaluated using KF-1 cells. KHV (KHV-I strain) was completely inactivated by UV irradiation at a dose of 4.0 × 10^3 μWs/cm^2 or heating to temperatures above 50℃ for 1 min. KHV was completely inactivated by 200 mg/L iodophor, 60 mg/L benzalkonium chloride solution or 30% ethyl alcohol for 20 min. When chlorine concentration was measured after mixing with virus supernatant containing medium and fetal bovine serum, 97.5% and 98.5% reduction in infectivity was observed at 0.30 mg/L of chlorine for 30 s and 20 min, respectively. In practice, a tenfold concentration of chlorine (i.e. 3 mg/L) will be recommended to inactivate KHV completely.
Type: article
URI: http://hdl.handle.net/2115/38365
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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