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Production of Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances by Immobilized Whole Living Cells of Aquatic Bacteria in a Bioreactor System

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Title: Production of Anti-Infectious Hematopoietic Necrosis Virus (IHNV) Substances by Immobilized Whole Living Cells of Aquatic Bacteria in a Bioreactor System
Authors: Myouga, Hisashi Browse this author
Yoshimizu, Mamoru Browse this author →KAKEN DB
Yumoto, Isao Browse this author
Ezura, Yoshio Browse this author
Kimura, Takahisa Browse this author
Keywords: antiviral substance
alginate beads
bioreactor
IHNV
Pseudomonas
Alteromonas
Issue Date: 1995
Publisher: Japanese Society of Fisheries Science
Journal Title: Fisheries Science
Volume: 61
Issue: 1
Start Page: 61
End Page: 64
Publisher DOI: 10.2331/fishsci.61.61
Abstract: A simple, low-cost and efficient bioreactor system for production of anti-infectious hematopoietic necrosis virus (IHNV) substances by bacteria isolated from aquatic environments was devised on a laboratory scale. Whole living cells of anti-IHNV substances producing bacteria, Pseudomonas sp. 51BBW-29 and Alteromonas sp. 48HS-27, were immobilized in calcium alginate beads prepared by 1% sodium alginate, 0.45M CaCl2, and C2Y or MC2Y broth. Eighteen g of the beads were packed into a 25 ml reaction column and incubated at 15℃ for 5 days in a circulating broth medium at a flow rate of 0.35ml/h. After activation of the immobilized bacteria, the beads were incubated in a continuous flow of fresh broth with intermittent exposure to air. In the beads incubated under these conditions, the immobilized bacteria grew well at the gel surface and high viability of approximately 10^10 CFU/bead was retained for at least 6 days from day 8 to 14. During 31 days of the experiment with the bioreactor system, the highest production was 69.3 ⊿IHD50 for Pseudomonas sp. 51BBW-29 (at 14-17 days incubation) and 48.0 ⊿IHD50 for Alteromonas sp. 48HS-27 (at 8-11 days incubation), which were 2.5 and 2.7 times higher than those of conventional batch culture (27.3 and 18.0 ⊿IHD50 for the two strains at 2-5 days incubation). The immobilized bacteria continuously produced high levels of anti-IHNV substances for 15 days (day 5-20).
Rights: © 1995 公益社団法人日本水産学会
© 1995 The Japanese Society of Fisheries Science
Type: article
URI: http://hdl.handle.net/2115/38579
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 吉水 守

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