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特異抗体感作 Staphylococci を用いた Coagglutination Test によるサケ科魚類の細菌性腎臓病 (BKD) の迅速診断法について

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タイトル: 特異抗体感作 Staphylococci を用いた Coagglutination Test によるサケ科魚類の細菌性腎臓病 (BKD) の迅速診断法について
その他のタイトル: Rapid Method for Detection of Bacterial Kidney Disease of Salmonid (BKD) by Coagglutination of Antibody Sensitized Protein A-Containing Staphylococci
著者: 木村, 喬久 著作を一覧する
吉水, 守 著作を一覧する
発行日: 1981年 9月
出版者: 日本水産学会 = The Japanese Society of Scientific Fisheries
誌名: 日本水産学会誌 = Bulletin of the Japanese Society of Scientific Fisheries
巻: 47
号: 9
開始ページ: 1173
終了ページ: 1183
出版社 DOI: 10.2331/suisan.47.1173
抄録: The application of a coagglutination test using staphylococci specifically sensitized with antibody against the bacterium causing bacterial kidney disease to the diagnosis of this disease in salmonids was studied. This method proved to be a simple, rapid and reliable diagnostic test suitable for use in the laboratory or field, and requires no special apparatus. It was found to be highly specific and more sensitive than the immunodiffusion test. In a study of 758 fish from 24 farms suspected in outbreaks of bacterial kidney disease the rate of detection of the disease with the coagglutination test was as high or higher than the rates obtained by Gram staining or clinical examination. The procedure of this method is summarized as follows: 1) Kidney sample from diseased fish is homogenized with four to nine volumes of PBS, and heated in a boiling water bath for 30min. 2) The supernatant is collected after centrifugation at 4000 rpm for 20min. This may be omitted if a centrifuge is unavailable. 3) One drop of the supernatant and one drop of anti-BKD antibody sensitized staphylococci suspension are mixed on a glass slide and incubated at room temperature. The slide is examined after 30, 60 and 120min. 4) If positive coagglutination is observed, the infected fish should be examined microscopically to confirm the diagnostic results.
最近開発された新しい血清学的診断法である特異抗体感作staphylococciを用いるcoagglutination test(共同凝集試験)の魚病診断,特にサケ科魚類の細菌性腎臓病 (BKD) 診断への応用について検討を行った結果,病魚患部加熱抽出液を抗原とするcoagglutination testにより,本病を現場において2~3時間の短時間でしかも特殊な実験装置を全く必要とせず,迅速且つ簡易に診断し得ること,さらに病魚材料の加熱処理試料を郵送することにより遠隔地での診断も可能であることを明らかにし得た。
Rights: ©1981 公益社団法人日本水産学会 The Japanese Society of Fisheries Science
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 吉水 守


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