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Susceptibilities against Bovine Lactoferrin with Microorganisms Isolated from Mastitic Milk

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Title: Susceptibilities against Bovine Lactoferrin with Microorganisms Isolated from Mastitic Milk
Other Titles: 乳房炎乳由来微生物のラクトフェリンに対する感受性
Authors: Lee, Nai-Yuan1 Browse this author
Kawai, Kazuhiro2 Browse this author
Nakamura, Ichiro3 Browse this author →KAKEN DB
Tanaka, Tetsuya4 Browse this author
Kumura, Haruto5 Browse this author →KAKEN DB
Shimazaki, Kei-ichi6 Browse this author
Authors(alt): 李, 乃元1
河合, 一洋2
中村, 一郎3
田仲, 哲也4
玖村, 朗人5
島崎, 敬一6
Keywords: antimicrobial activity
mastitic milk
Issue Date: 25-Oct-2004
Publisher: 日本獣医学会
Journal Title: The Journal of Veterinary Medical Science
Volume: 66
Issue: 10
Start Page: 1267
End Page: 1269
Abstract: 臨床型乳房炎乳からE. coli(11株),K. pneumoniae(5株),enterococci(8株), S. aureus(10株),CNS(13株),streptococci(11株),P. zopfii(7株),yeast-like fungi(9株)を分離し,ラクトフェリンに対する感受性を比較した.その結果,P. zopfiiがラクトフェリンによって最も増殖抑制を受け易かった.一方,E. coliやenterococciはラクトフェリンの影響を受けにくく,またS. aureusは菌株によって感受性の有無が分かれた.
Antibacterial effects of bovine lactoferrin were studied in vitro against microorganisms isolated from mastitic milk in Tokachi area, Hokkaido, Japan. Microorganisms isolated were Eschelichia coli (11 isolates), Klebsiella pneumoniae (5 isolates), enterococci (8 isolates), Staphylococcus aureus (10 isolates), coagulase negative staphylococci (CNS, 13 isolates), streptococci (11 isolates), Prototheca zopfii (7 isolates) and yeast-like fungi (9 isolates). Lactoferrin has been known as a multifunctional protein and its antimicrobial effect is one of the most essential function of it. In order to compare their susceptibilities against lactoferrin, the minimal inhibitory concentration values were estimated by a microplate assay method using 96-well microplate, which involved measuring the optical density of the cultures. Prototheca zopfii was highly sensitive to bovine lactoferrin and complete inhibition of this microorganism was observed even at the low concentration of 7 μg/ml. On the other hand, E. coli and enterococci showed resistance against lactoferrin action and staphylococci showed strain-dependent resistance.
Rights: 本文データは学協会の許諾に基づきCiNiiから複製したものである日本獣医学会
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 島崎 敬一

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