HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
薬学研究院  >
雑誌発表論文等  >

Targeted sequence alteration of a chromosomal locus in mouse liver

IJP387-1-2_180-183.pdf373.23 kBPDF見る/開く

タイトル: Targeted sequence alteration of a chromosomal locus in mouse liver
著者: Kamiya, Hiroyuki 著作を一覧する
Uchiyama, Masayuki 著作を一覧する
Piao, Jingshu 著作を一覧する
Nakatsu, Yoshimichi 著作を一覧する
Tsuzuki, Teruhisa 著作を一覧する
Harashima, Hideyoshi 著作を一覧する
キーワード: Targeted sequence alteration
Gene correction
Tailed duplex DNA fragment
rpsL transgenic mouse
Hydrodynamic tail vein injection
発行日: 2010年 3月15日
出版者: Elsevier B.V.
誌名: International Journal of Pharmaceutics
巻: 387
号: 1-2
開始ページ: 180
終了ページ: 183
出版社 DOI: 10.1016/j.ijpharm.2009.12.020
抄録: Targeted sequence alteration would be an attractive method in gene therapy and biotechnology. To achieve in vivo targeted sequence alteration, a tailed duplex DNA consisting of annealed 35mer and 794mer single-stranded DNAs was delivered by means of hydrodynamic tail vein injection into liver of transgenic mouse harboring a reporter gene (the rpsL gene) in its genome. The tailed DNA was designed for a conversion of ATC to AGC at codon 80 of the rpsL transgene. The anticipated T → G sequence alteration was induced in the transgene in the liver with an efficiency of ∼0.1%. These results demonstrate the significant potential of this method for applications in gene therapy and biotechnology.
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 紙谷 浩之


本サイトに関するご意見・お問い合わせは repo at へお願いします。 - 北海道大学