Targeted sequence alteration of a chromosomal locus in mouse liver

Kamiya, Hiroyuki; Uchiyama, Masayuki; Piao, Jingshu; Nakatsu, Yoshimichi; Tsuzuki, Teruhisa; Harashima, Hideyoshi

doi:10.1016/j.ijpharm.2009.12.020


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Targeted sequence alteration of a chromosomal locus in mouse liver

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/42890

Title:	Targeted sequence alteration of a chromosomal locus in mouse liver
Authors:	Kamiya, Hiroyuki Browse this author
	Uchiyama, Masayuki Browse this author
	Piao, Jingshu Browse this author
	Nakatsu, Yoshimichi Browse this author
	Tsuzuki, Teruhisa Browse this author
	Harashima, Hideyoshi Browse this author →KAKEN DB
Keywords:	Targeted sequence alteration
	Gene correction
	Tailed duplex DNA fragment
	rpsL transgenic mouse
	Hydrodynamic tail vein injection
Issue Date:	15-Mar-2010
Publisher:	Elsevier B.V.
Journal Title:	International Journal of Pharmaceutics
Volume:	387
Issue:	1-2
Start Page:	180
End Page:	183
Publisher DOI:	10.1016/j.ijpharm.2009.12.020
PMID:	20025952
Abstract:	Targeted sequence alteration would be an attractive method in gene therapy and biotechnology. To achieve in vivo targeted sequence alteration, a tailed duplex DNA consisting of annealed 35mer and 794mer single-stranded DNAs was delivered by means of hydrodynamic tail vein injection into liver of transgenic mouse harboring a reporter gene (the rpsL gene) in its genome. The tailed DNA was designed for a conversion of ATC to AGC at codon 80 of the rpsL transgene. The anticipated T → G sequence alteration was induced in the transgene in the liver with an efficiency of ∼0.1%. These results demonstrate the significant potential of this method for applications in gene therapy and biotechnology.
Type:	article (author version)
URI:	http://hdl.handle.net/2115/42890
Appears in Collections:	薬学研究院 (Faculty of Pharmaceutical Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 紙谷浩之

OAI-PMH ( junii2 , jpcoar_1.0 )

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