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Detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes

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Title: Detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes
Other Titles: Fibrobacter succinogenesを中心とするルーメン内繊維分解コンソーシャム構成細菌の検出および同定
Authors: Shinkai, Takumi1 Browse this author
Ueki, Takaaki2 Browse this author
Kobayashi, Yasuo3 Browse this author →KAKEN DB
Authors(alt): 真貝, 拓三1
上木, 孝亮2
小林, 泰男3
Keywords: 16S rRNA gene
bacterial consortium
fiber digestion
Fibrobacter succinogenes
rumen bacteria
Issue Date: Feb-2010
Publisher: Blackwell Publishing
Journal Title: Animal Science Journal
Volume: 81
Issue: 1
Start Page: 72
End Page: 79
Publisher DOI: 10.1111/j.1740-0929.2009.00698.x
PMID: 20163675
Abstract: A fibrolytic consortium, dominated by the rumen cellulolytic bacterium Fibrobacter succinogenes, was artificially constructed on hay stems to detect and identify rumen bacteria that can potentially interact with F. succinogenes. Consortium-bacterial members were determined by DGGE and sequencing analysis targeted bacterial 16S rDNA. An artificial consortium was formed in a 2-step incubation of hay stems; the first step with group 1, 2 or 3 F. succinogenes strains, the second step with rumen fluid. After consortium formation, morphologically different bacteria were observed in association with F. succinogenes. DGGE exhibited more than 30 bands, the pattern of which depended on the F. succinogenes group. Sequencing suggested that Butyrivibrio fibrisolvens, Pseudobutyrivibrio ruminis, Clostridium sp., F. succinogenes group 2, Prevotella ruminicola and unclassified Bacteroides were prominent in the group 1 consortium and that Treponema bryantii, B. fibrisolvens, Acinetobacter sp, and Wolinella succinogenes were prominent in the group 2 consortium. However, in the group 3 consortium, F. succinogenes-like bacteria were microscopically undetectable, whereas cellulolytic Ruminococcus albus and F. succinogenes group 1 were prominent, suggesting that the group 3 cannot be a core member of this consortium. This study is the first attempt to identify bacterial members of a fibrolytic consortium dominated by a specific bacterium.
ルーメン内繊維分解性細菌Fibrobacter succinogenesと相互作用をもつ細菌種を検出・同定するため、本菌系統グループ1から3の菌株を中心とする繊維分解コンソーシャムをオーチャードグラス乾草上に人為的に作成した(作成コンソーシャム)。作成コンソーシャムにおいてはF. succinogenes様細胞に異なる形態の細菌が付着する様子が電顕観察された。作成コンソーシャムのDGGE解析では30以上のバンドが検出され、そのバンドパターンは主構成員である系統グループの違いを反映していた。グループ1の作成コンソーシャムからはButyrivibrio fibrisolvens, Pseudobutyrivibrio ruminis, Clostridium属細菌, F. succinogenesグループ2, Prevotella ruminicolaおよび未培養のBacteroides属細菌が、グループ2のそれからはTreponema bryantii, B. fibrisolvens, Acinetobacter属細菌およびWolinella succinogenesが構成細菌として同定された。一方、グループ3のコンソーシャムでは主構成員であるはずのF. succinogenesの観察頻度は下がり、他の繊維分解菌に置き換わっていたため、グループ3のF. succinogenesはコンソーシャムで主導的な役割を担い得ないと考えられた。本研究は特定の細菌を中心として構成される繊維分解コンソーシャムに対し、その構成細菌を明らかにしようとした初めての報告である。
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Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 泰男

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