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Differential expression of the auxin primary response gene MASSUGU2/IAA19during tropic responses of Arabidopsis hypocotyls

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Title: Differential expression of the auxin primary response gene MASSUGU2/IAA19during tropic responses of Arabidopsis hypocotyls
Authors: Saito, Kyoko Browse this author
Watahiki, Masaaki K. Browse this author
Yamamoto, Kotaro T. Browse this author
Issue Date: 4-Apr-2007
Publisher: Blackwell Publishing
Journal Title: Physiologia Plantarum
Volume: 130
Issue: 1
Start Page: 148
End Page: 156
Publisher DOI: 10.1111/j.1399-3054.2007.00898.x
Abstract: We have examined the expression pattern of an auxin primary response gene, MSG2/IAA19, during photo- and gravitropic responses of hypocotyls using a transgenic Arabidopsis harboring MSG2/IAA19 promoter::GUS. The upper portion of most etiolated hypocotyls showed uniform b-glucuronidase (GUS) staining with the strongest activity in the pericycle. When hypocotyls were irradiated with unilateral blue light, GUS activity on the concave side of hypocotylswas decreased, resulting in differentialGUSstaining with a stronger signal on the convex side. The number of differentially stained hypocotyls peaked at 24 h after the onset of the phototropic stimuli, while hypocotyl curvature continued to increase for the entire 36-h experimental period. This result suggests that the MSG2/IAA19 expression precedes the phototropic responses. When seedlings were grown under dim white light, their hypocotyls displayed almost no GUS activity. The light-grown hypocotyls also showed differential GUS staining after phototropic stimuli as result of the increase in GUS activity on the convex side of hypocotyls, especially in the epidermis, the outer cortex and pericycle, although GUS activity was much weaker than that observed in etiolated hypocotyls. Similar but less obvious differential staining was obtained for gravitropic response of hypocotyls. Considering the recent finding thatAux/IAA proteins are immediate targets of the auxin F box receptors, MSG2/IAA19 is likely to act as one of master genes for tropic responses.
Rights: The definitive version is available at www.blackwell-synergy.com
Type: article (author version)
URI: http://hdl.handle.net/2115/44829
Appears in Collections:理学院・理学研究院 (Graduate School of Science / Faculty of Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山本 興太朗

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