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Immunohistochemical observations of vitellin synthesis and accumulation processes in ovary of Ezo abalone Haliotis discus hannai

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Title: Immunohistochemical observations of vitellin synthesis and accumulation processes in ovary of Ezo abalone Haliotis discus hannai
Other Titles: エゾアワビ卵巣におけるビテリンの産生と蓄積過程の免疫組織学的解析
Authors: Awaji, Masahiko1 Browse this author
Matsumoto, Toshie2 Browse this author
Yamano, Keisuke3 Browse this author
Kitamura, Makiko4 Browse this author
Hara, Akihiko5 Browse this author →KAKEN DB
Authors(alt): 淡路, 雅彦1
松本, 才絵2
山野, 恵祐3
北村, 真紀子4
原, 彰彦5
Keywords: Vitellin
Vitellogenin
Follicle cells
Abalone
Mollusk
Issue Date: Mar-2011
Publisher: Springer Japan
Journal Title: Fisheries Science
Volume: 77
Issue: 2
Start Page: 191
End Page: 197
Publisher DOI: 10.1007/s12562-010-0316-5
Abstract: Ovarian follicle cells are the site of yolk protein synthesis in the Ezo abalone Haliotis discus hannai. In this study, histological observations of ovarian follicle cells were conducted in H. discus hannai by immunocytochemical and in situ hybridization methods focusing on their function of yolk protein synthesis. An antibody raised against purified yolk protein (vitellin, Vn) recognized yolk granules in oocytes, and ovarian follicle cells adjacent to the oocytes under yolk accumulation were stained positively with both anti-Vn antibody and an antisense probe for vitellogenin (the precursor for vitellin, Vtg) mRNA. These results indicate that the abalone Vtg gene is transcribed and translated in the ovarian follicle cells. In oocytes in the early phase of yolk accumulation, positive reactions with the antibody appeared first in the stalk part, and the follicle cells adjacent to the stalk were also stained positively. These observations imply local transportation of yolk protein from the follicle cells to the oocyte through an extracellular space around the oocyte stalk. Ovarian follicle cells changed their morphological characteristics and reactivity to the antibody with close relationships with the stages of the adjacent oocyte, which suggests the presence of functional interactions between follicle cells and oocytes in the course of yolk protein synthesis and accumulation.
エゾアワビ卵巣濾胞細胞(濾胞細胞)の卵黄タンパク質(ビテリン、Vn)合成と卵母細胞へのVn蓄積過程について、抗Vn抗体による免疫染色とVn前駆体遺伝子のin situ ハイブリダイゼーションで組織学的に検討した。その結果、前駆体遺伝子が濾胞細胞で転写、翻訳されることが示され、合成された卵黄タンパク質は濾胞細胞と卵母細胞が接する卵柄部で卵母細胞内に輸送されると示唆された。また濾胞細胞の形態と抗Vn抗体への反応性は隣接する卵母細胞の発達と密接に関連し、Vn合成と蓄積において濾胞細胞と卵母細胞間に相互作用が存在すると推定された。
Rights: © 2011 公益社団法人日本水産学会
© 2011 The Japanese Society of Fisheries Science
Type: article (author version)
URI: http://hdl.handle.net/2115/45041
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 原 彰彦

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