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Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli
Title: | Lactate fraction dependent mechanical properties of semitransparent poly(lactate-co-3-hydroxybutyrate)s produced by control of lactyl-CoA monomer fluxes in recombinant Escherichia coli |
Authors: | Yamada, Miwa Browse this author | Matsumoto, Ken'ichiro Browse this author →KAKEN DB | Uramoto, Shu Browse this author | Motohashi, Ren Browse this author | Abe, Hideki Browse this author | Taguchi, Seiichi Browse this author →KAKEN DB |
Keywords: | Lactate-based polyester | Lactate-polymerizing enzyme | Poly(lactic acid) | Polyhydroxyalkanoate | Lactic acid |
Issue Date: | 20-Jul-2011 |
Publisher: | Elsevier B.V. |
Journal Title: | Journal of Biotechnology |
Volume: | 154 |
Issue: | 4 |
Start Page: | 255 |
End Page: | 260 |
Publisher DOI: | 10.1016/j.jbiotec.2011.05.011 |
PMID: | 21640144 |
Abstract: | In order to evaluate the mechanical properties of poly(lactate-co-3-hydroxybutyrate) [P(LA-co-3HB)] and its correlation with the LA fraction, P(LA-co-3HB)s with a variety of LA fractions were prepared using recombinant Escherichia coli expressing the LA-polymerizing enzyme and monomer supplying enzymes. The LA-overproducing mutant E. coli JW0885 with a pflA gene disruption was used for the LA-enriched polymer production. The LA fraction was also varied by jar-fermentor based fine-regulation of the anaerobic status of the culture conditions, resulting in LA fractions ranging from 4 to 47 mol%. In contrary to the opaque P(3HB) film, the copolymer films attained semitransparency depending on the LA fraction. Young's modulus values of the P(LA-co-3HB)s (from 148 to 905 MPa) were lower than those of poly(lactic acid) (PLA) (1020 MPa) and P(3HB) (1079 MPa). In addition, the values of elongation at break of the copolymer with 29 mol% LA reached 150%. In conclusion, P(LA-co-3HB)s were found to be a comparatively pliable and flexible material, differing from both of the rigid homopolymers. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/47101 |
Appears in Collections: | 工学院・工学研究院 (Graduate School of Engineering / Faculty of Engineering) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 松本 謙一郎
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