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Hokkaido University Collection of Scholarly and Academic Papers >
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Molecular Pathology of Murine Ureteritis Causing Obstructive Uropathy with Hydronephrosis
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| Title: | Molecular Pathology of Murine Ureteritis Causing Obstructive Uropathy with Hydronephrosis |
| Authors: | Ichii, Osamu Browse this author →KAKEN DB | | Otsuka, Saori Browse this author →KAKEN DB | | Namiki, Yuka Browse this author →KAKEN DB | | Hashimoto, Yoshiharu Browse this author →KAKEN DB | | Kon, Yasuhiro Browse this author →KAKEN DB |
| Issue Date: | 16-Nov-2011 |
| Publisher: | Public Library of Science |
| Journal Title: | PLoS One |
| Volume: | 6 |
| Issue: | 11 |
| Start Page: | e27783 |
| Publisher DOI: | 10.1371/journal.pone.0027783 |
| Abstract: | Primary causes of urinary tract obstruction that induces urine retention and results in hydronephrosis include uroliths, inflammation, and tumors. In this study, we analyzed the molecular pathology of ureteritis causing hydronephrosis in laboratory rodents. F2 progenies of C57BL/6 and DBA/2 mice were studied histopathologically and by comprehensive gene expression analysis of their ureters. Incidence of hydronephrosis was approximately 5% in F2 progenies. Histopathologically, this hydronephrosis was caused by stenosis of the proximal ureter, which showed fibrosis and papillary malformations of the proliferative epithelium with infiltrations of B-cell-dominated lymphocytes. Additionally, CD16-positive large granular leukocytes and eosinophils infiltrated from the ureteral mucosa to the muscular layer. Eosinophilic crystals were characteristically observed in the lumen of the ureter and the cytoplasm of large granular leukocytes, eosinophils, and transitional epithelial cells. Comprehensive gene profiling revealed remarkably elevated expression of genes associated with hyperimmune responses through activation of B cells in diseased ureters. Furthermore, diseased ureters showed dramatically higher gene expression of chitinase 3-like 3, known as Ym1, which is associated with formation both of adenomas in the transitional epithelium and of eosinophilic crystals in inflammatory conditions. The Ym1 protein was mainly localized to the cytoplasm of the transitional epithelium, infiltrated cells, and eosinophilic crystals in diseased ureters. We determined that the primary cause of hydronephrosis in F2 mice was ureteritis mediated by the local hyperimmune response with malformation of the transitional epithelium. Our data provide a novel molecular pathogenesis for elucidating causes of aseptic inflammation in human upper urinary tracts. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/47961 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 市居 修
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