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北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University >
第62巻 第1号 >


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Title: 沿岸域海底泥中に存在する珪藻類休眠期細胞の凍結耐性
Other Titles: Cryopreservation tolerance of Marine Diatom Resting Stage Cells in the Coastal Sea Bottom Sediments
Authors: 板倉, 茂1 Browse this author →KAKEN DB
長崎, 慶三2 Browse this author →KAKEN DB
河村, 知彦3 Browse this author →KAKEN DB
今井, 一郎4 Browse this author →KAKEN DB
Authors(alt): Itakura, Shigeru1
Nagasaki, Keizo2
Kawamura, Tomohiko3
Imai, Ichiro4
Keywords: Diatom
Resting stage cells
Coastal bottom sediment
Thalassiosira pseudonana
Nitzschia sp.
Issue Date: 28-Mar-2012
Publisher: 北海道大学大学院水産科学研究院
Journal Title: 北海道大学水産科学研究彙報
Journal Title(alt): Bulletin of Fisheries Sciences, Hokkaido University
Volume: 62
Issue: 1
Start Page: 15
End Page: 19
Abstract: In spite of its importance in microbiology, information on cryopreservation of marine diatom is limited. An attempt was made to develop cryopreservation procedure of marine diatom resting stage cells which abundantly exist in the bottom sediment of coastal area. Bottom sediment samples from the Seto Inland Sea (Harima-Nada) were used for sub-zero temperature preservation experiment in this study. The condition giving optimal survival of microalgae in cryopreserved sediments was as follows : with sorbitol (1 M) as additives, 1/3 salinity, at -80°C preservation (no pre-cooling). Under this condition, two diatoms, Thalassiosira pseudonana and Nitzschia sp., and some unidentified microalgae were recovered from the sediments, but the vegetative cells of obtained diatoms were not able to be cryopreserved again under the same conditions. Present result suggests that these two diatoms were preserved as resting stage cells in the coastal bottom sediments, and the diatom resting stage cells might be more resistant to cryopreservation than the vegetative cells.
Type: bulletin (article)
Appears in Collections:北海道大学水産科学研究彙報 = Bulletin of Fisheries Sciences, Hokkaido University > 第62巻 第1号

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