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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Phenotypic grouping of 141 BmNPVs lacking viral gene sequences
| Title: | Phenotypic grouping of 141 BmNPVs lacking viral gene sequences |
| Authors: | Ono, Chikako Browse this author | | Kamagata, Takanori Browse this author | | Taka, Hitomi Browse this author | | Sahara, Ken Browse this author →KAKEN DB | | Asano, Shin-ichiro Browse this author →KAKEN DB | | Bando, Hisanori Browse this author →KAKEN DB |
| Keywords: | BmNPV | | Bacmid | | Lambda red recombination system | | Gene knockout |
| Issue Date: | May-2012 |
| Publisher: | Elsevier B.V. |
| Journal Title: | Virus Research |
| Volume: | 165 |
| Issue: | 2 |
| Start Page: | 197 |
| End Page: | 206 |
| Publisher DOI: | 10.1016/j.virusres.2012.02.016 |
| PMID: | 22421381 |
| Abstract: | We constructed a series of gene knockout BmNPVs (KOVs) for each of 141 genes using the BmNPV T3 bacmid system and lambda red recombination system. In a subsequent analysis of the properties needed for infection using a marker gene, egfp (enhanced green fluorescent protein gene), inserted into the polyhedrin locus, the knockout viruses (KOVs) were subdivided into four phenotypic types, A to D. Type-A (86 KOVs) showed the ability to expand infections equivalent to the control while type-B (8 KOVs) spread infections more slowly. Type-C (37 KOVs) expressed egfp in transfected-BmN cells but the production of infectious viruses was not observed. Type-D (10 KOVs) showed no ability to express egfp even in the transfection experiments. KOVs lacking genes (pkip (Bm15), gp41 (Bm66), bro-d (Bm131), Bm20, 48, 65, 91, 93, or 101) previously identified as being essential, were placed in the viable type-A and B categories. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/49265 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 伴戸 久徳
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