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Phenotypic grouping of 141 BmNPVs lacking viral gene sequences

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Title: Phenotypic grouping of 141 BmNPVs lacking viral gene sequences
Authors: Ono, Chikako Browse this author
Kamagata, Takanori Browse this author
Taka, Hitomi Browse this author
Sahara, Ken Browse this author →KAKEN DB
Asano, Shin-ichiro Browse this author →KAKEN DB
Bando, Hisanori Browse this author →KAKEN DB
Keywords: BmNPV
Bacmid
Lambda red recombination system
Gene knockout
Issue Date: May-2012
Publisher: Elsevier B.V.
Journal Title: Virus Research
Volume: 165
Issue: 2
Start Page: 197
End Page: 206
Publisher DOI: 10.1016/j.virusres.2012.02.016
PMID: 22421381
Abstract: We constructed a series of gene knockout BmNPVs (KOVs) for each of 141 genes using the BmNPV T3 bacmid system and lambda red recombination system. In a subsequent analysis of the properties needed for infection using a marker gene, egfp (enhanced green fluorescent protein gene), inserted into the polyhedrin locus, the knockout viruses (KOVs) were subdivided into four phenotypic types, A to D. Type-A (86 KOVs) showed the ability to expand infections equivalent to the control while type-B (8 KOVs) spread infections more slowly. Type-C (37 KOVs) expressed egfp in transfected-BmN cells but the production of infectious viruses was not observed. Type-D (10 KOVs) showed no ability to express egfp even in the transfection experiments. KOVs lacking genes (pkip (Bm15), gp41 (Bm66), bro-d (Bm131), Bm20, 48, 65, 91, 93, or 101) previously identified as being essential, were placed in the viable type-A and B categories.
Type: article (author version)
URI: http://hdl.handle.net/2115/49265
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 伴戸 久徳

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