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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Life Science / Faculty of Advanced Life Science >
Peer-reviewed Journal Articles, etc >
New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes.
| Title: | New evidence for the involvement of prostaglandin receptor EP4b in ovulation of the medaka, Oryzias latipes. |
| Authors: | Fujimori, Chika Browse this author | | Ogiwara, Katsueki Browse this author →KAKEN DB | | Hagiwara, Akane Browse this author | | Takahashi, Takayuki Browse this author →KAKEN DB |
| Keywords: | Prostaglandin E2 | | EP receptor | | Intracellular cAMP level | | Gene expression | | Ovulation | | Medaka |
| Issue Date: | 15-Oct-2012 |
| Publisher: | Elsevier Science |
| Journal Title: | Molecular and cellular endocrinology |
| Volume: | 362 |
| Issue: | 1-2 |
| Start Page: | 76 |
| End Page: | 84 |
| Publisher DOI: | 10.1016/j.mce.2012.05.013 |
| PMID: | 22659410 |
| Abstract: | A cDNA for a prostaglandin E(2) (PGE(2)) receptor subtype 4, EP4b (Ptger4b), was cloned from the medaka ovary. The effect of PGE(2) was examined using COS-7 cells expressing the recombinant Ptger4b protein. An increase in intracellular cAMP levels was observed when the cells were incubated with PGE(2), but the increase in cAMP levels was nullified by the addition of the EP4 antagonist GW627368X. The expression of ptger4b mRNA was drastically induced by the addition of pregnant mare serum gonadotropin to the in vitro culture of large preovulatory follicles. In in vitro ovulation studies of the effect of GW627368X addition on follicle ovulation, the critical timing of the PGE(2)/Ptger4b interaction was suggested to be between -1 and 0h of ovulation. These results further substantiate that PGE(2)/Ptger4b signaling is involved in follicle rupture during ovulation in the medaka ovary. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/49982 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荻原 克益
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