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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
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Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression.
| Title: | Glutamate transporter GLT-1 is transiently localized on growing axons of the mouse spinal cord before establishing astrocytic expression. |
| Authors: | Yamada, K. Browse this author | | Watanabe, M. Browse this author | | Shibata, T. Browse this author | | Nagashima, M. Browse this author | | Tanaka, K. Browse this author | | Inoue, Y. Browse this author |
| Issue Date: | 1-Aug-1998 |
| Publisher: | Society for Neuroscience |
| Journal Title: | The Journal of neuroscience : the official journal of the Society for Neuroscience |
| Volume: | 18 |
| Issue: | 15 |
| Start Page: | 5706 |
| End Page: | 5713 |
| Publisher DOI: | 10.1523/JNEUROSCI.18-15-05706.1998 |
| PMID: | 9671661 |
| Abstract: | The glutamate transporter GLT-1 is expressed in astrocytes of the mature brain and spinal cord. In the present study, we examined its expression in the developing mouse spinal cord. By in situ hybridization, 35S-labeled antisense oligonucleotide probes for GLT-1 mRNA consistently labeled the mantle zone/gray matter from embryonic day 11 through the adult stage. However, immunohistochemistry with a specific antibody visualized distinct regional and cellular localizations during the time between the fetal and postnatal stages. At fetal stages, GLT-1 immunoreactivity predominated in the marginal zone/white matter, observed as tiny puncta in cross-sections and as thin fibers in longitudinal sections. The GLT-1-immunopositive structures were also labeled for neuron-specific enolase, a glycolytic enzyme specific to postmitotic neurons and endocrine cells. By electron microscopy, GLT-1 immunoreactivity was detected in axons forming frequent enlargements and was focally localized on a small portion of the axolemma, particularly that facing adjacent axons. At early postnatal stages, GLT-1 disappeared from axons in white matter tracts and, instead, appeared in astrocytic processes surrounding various neuronal elements in the gray matter. Therefore, before switching to astrocytic expression, GLT-1 is transiently expressed in neurons and localized in differentiating axons. Together with our previous finding on the localization of glutamate transporter GLAST in radial glial fibers, GLT-1 and GLAST are thus localized during development on distinct directional cellular elements along which young neurons elongate their axons or move their cell bodies, respectively. |
| Relation: | http://www.jneurosci.org/content/18/15/5706.full.pdf+html |
| Type: | article |
| URI: | http://hdl.handle.net/2115/51750 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 渡邉 雅彦
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